Xiaowen Liu

US Patent:

7754467, Jul 13, 2010

Filed:

Jan 28, 2004

Appl. No.:

10/766993

Inventors:

Chia-Hwa Chang - Mountain View CA, US
Xiaowen Liu - Cupertino CA, US
John A. Lewicki - Los Gatos CA, US
Qiang Xu - Cupertino CA, US

Assignee:

Osel, Inc. - Santa Clara CA

International Classification:

C12N 1/21
A61K 48/00
C07K 14/195

US Classification:

4352523, 424 932, 530350

Abstract:

Methods and compositions for targeting heterologous polypeptides to bacterial cell walls are provided.

US Patent:

20060194226, Aug 31, 2006

Filed:

Jan 12, 2006

Appl. No.:

11/331965

Inventors:

Xiaowen Liu - Cupertino CA, US
Kirsten Essenmacher - Palo Alto CA, US
David Simpson - Redwood City CA, US
Qiang Xu - Cupertino CA, US

Assignee:

Osel, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435006000, 435007100

Abstract:

This disclosure relates to a modified version of cyanovirin-N protein and a transformed bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed bacterium. In addition, this disclosure describes two novel promoters originated from which are capable of directing a high level of gene expression in bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified bacterium containing the expression cassette, a method for recombinant gene expression in bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.

US Patent:

20090155304, Jun 18, 2009

Filed:

Nov 24, 2008

Appl. No.:

12/277193

Inventors:

Xiaowen Liu - Cupertino CA, US
Kirsten Essenmacher - Palo Alto CA, US
David A. Simpson - Redwood City CA, US
Qiang Xu - Cupertino CA, US

Assignee:

Osel, Inc. - Santa Clara CA

International Classification:

A61K 39/12
C12N 1/21
C07K 14/00
C07K 5/00
C07K 7/00
A61K 35/74
C12N 15/11
A61K 38/16
C12N 15/00
C12P 21/02

US Classification:

4242001, 4352523, 530350, 530330, 530329, 424 932, 536 237, 514 12, 4353201, 435 691

Abstract:

This disclosure relates to a modified version of cyanovirin-N protein and a transformed bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed bacterium. In addition, this disclosure describes two novel promoters originated from , which are capable of directing a high level of gene expression in bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified bacterium containing the expression cassette, a method for recombinant gene expression in bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.

US Patent:

20220356537, Nov 10, 2022

Filed:

Dec 28, 2020

Appl. No.:

17/789652

Inventors:

- Pleasanton CA, US
Xiaowen Liu - Cupertino CA, US
Marc Rehfuss - Alameda CA, US
Jingtao Sun - San Ramon CA, US
Matthew Youngblut - Berkeley CA, US
Rui Zhang - Dublin CA, US

International Classification:

C12Q 1/70

Abstract:

The present invention relates to methods and compositions for performing quantitative polymerase chain reaction (qPCR) to screen for previously undiscovered inducible prophages from various bacterial strains. The present invention also relates to methods for performing qPCR to identify inducible prophages for creation of functional non-replicative transcription particles (NRTPs).

US Patent:

20170166907, Jun 15, 2017

Filed:

Feb 16, 2017

Appl. No.:

15/434937

Inventors:

- Los Gatos CA, US
Marc Rehfuss - Alameda CA, US
Xiaowen Liu - Cupertino CA, US

International Classification:

C12N 15/70
C12N 9/02
C07K 14/005
C12N 7/00
C12Q 1/68

Abstract:

Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.