Cheng Li - Los Angeles CA Wing Hung Wong - Los Angeles CA
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
G06K 900
US Classification:
382133
Abstract:
A characteristic intensity of a feature in image data generated by scanning a microarray probe is determined. A set of pixels of the image data that nominally represent the feature is identified. The pixels each have an value (such as an intensity value) associated therewith. For each of a plurality of subsets of the set of pixels, a variation statistic value is determined that corresponds to a variation in the values associated with the pixels of that subset. One of the subsets of pixels is chosen based on the determined variation statistic values. A method is also described to relate a first expression array of probes to a second expression array of probes. A subset of the probe for the arrays is determined based on a comparison of the ordering of the subset of the probes of the second array, according to a particular characteristic of the probes, to the ordering of corresponding probes in the first array according to the particular characteristic of the probes. A relationship of the second expression array to the first expression array is determined based on the subset of probes o f the second expression array to the corresponding probes of the first array.
The invention is directed to a method for sequencing multiple target polynucleotide segments in parallel, and to compositions and kits therefor. In the method, a plurality of sample polynucleotide fragments are used to form a mixture of different-length sequencing fragments. The sequencing fragments are complementary to at least two different sample fragments, wherein (1) each sequencing fragment terminates at a predefined end with a known base or bases, and (2) each sequencing fragment contains an identifier tag sequence that identifies the sample fragment to which the sequencing fragment corresponds. The sequencing fragments are then separated on the basis of size to produce a plurality of resolved, size-separated bands. Resolved bands are collected in separate aliquots, which, in a preferred embodiment, are then subjected to an amplification step to amplify the complements of the tag sequences in each aliquot, and preferably, the tag sequences too. Amplification is preferably by PCR.
Wing Chai Wong - Brooklyn NY, US Pik Ngar Wong - Brooklyn NY, US
International Classification:
B23B 51/04
US Classification:
408 67, 408204
Abstract:
The slug retrieval apparatus is used with a hole saw with chuck mechanism, after a pilot hole is drilled. The apparatus is fitted within the chuck mechanism and the hole saw then used to cut a hole in the workpiece. The shaft of the apparatus guides the hole saw cut, as would the shaft of a pilot bit. The springs within the slot of the shaft compress within the slot to allow the shaft to pass through the pilot hole drilled in the workpiece. The springs then expand to capture the shaft within the hole. When the slug is cut from the workpiece by the saw, the springs capture the slug until the user compresses the springs to remove the slug. The springs provide for expansion no matter the speed of the apparatus used to drive the hole saw.
Parallel Polynucleotide Sequencing Method Using Tagged Probes.
The invention is directed to a method for sequencing multiple target polynucleotide segments in parallel, and to compositions and kits therefor. In the method, a plurality of sample polynucleotide fragments are used to form a mixture of different-length sequencing fragments. The sequencing fragments are complementary to at least two different sample fragments, wherein (1) each sequencing fragment terminates at a predefined end with a known base or bases, and (2) each sequencing fragment contains an identifier tag sequence that identifies the sample fragment to which the sequencing fragment corresponds. The sequencing fragments are then separated on the basis of size to produce a plurality of resolved, size-separated bands. Resolved bands are collected in separate aliquots, which, in a preferred embodiment, are then subjected to an amplification step to amplify the complements of the tag sequences in each aliquot, and preferably, the tag sequences too. Amplification is preferably by PCR. The (amplified) aliquots are then separately hybridized with an array of immobilized different-sequence tag probes under conditions effective to provide specific hybridization of tag sequences, or of tag sequence complements, with the corresponding immobilized tag probes, to form a hybridization pattern on the array, from which sequence information of one or more sample fragments are determined.
Parallel Polynucleotide Sequencing Method Using Tagged Primers
The Chinese University of Hong Kong - New Territories
International Classification:
C12Q 168 C12P 1934 C07H 2104
US Classification:
435 6
Abstract:
The invention is directed to a method for sequencing multiple target polynucleotide segments in parallel, and to compositions and kits therefor. In the method, a plurality of sample polynucleotide fragments are used to form a mixture of different-length sequencing fragments. The sequencing fragments are complementary to at least two different sample fragments, wherein (1) each sequencing fragment terminates at a predefined end with a known base or bases, and (2) each sequencing fragment contains an identifier tag sequence that identifies the sample fragment to which the sequencing fragment corresponds. The sequencing fragments are then separated on the basis of size to produce a plurality of resolved, size-separated bands. Resolved bands are collected in separate aliquots, which, in a preferred embodiment, are then subjected to an amplification step to amplify the complements of the tag sequences in each aliquot, and preferably, the tag sequences too. Amplification is preferably by PCR.
Method Of Translating Free-Format Data Records Into A Normalized Format Based On Weighted Attribute Variants
Mooi C. Chuah - Middletown NJ Wing S. Wong - Holmdel NJ
Assignee:
AT&T Corp. - Murray Hill NJ
International Classification:
G06F 1730
US Classification:
395600
Abstract:
A facility is provided for normalizing the format of stored data records using a dictionary that is generated from a training set of data records having predefined formats.
Americare Brooklyn, NY Jun 2010 to Dec 2010 Service CoordinatorHamilton-Madison House New York, NY Jan 2008 to Oct 2008 Special Outreach Worker/CounselorOffice AssistantNew York, NY Jan 2000 to Jan 2001
Education:
Brooklyn College, City University of New York 2012 Bachelor in AccountancyCollege at Old Westbury, State University of New York Dec 2006 Bachelor in Chemistry