Weilei Ma

US Patent:

20060154320, Jul 13, 2006

Filed:

Jan 6, 2006

Appl. No.:

11/326689

Inventors:

Robert Zuk - Atherton CA, US
Sae Choo - San Jose CA, US
Weilei Ma - Palo Alto CA, US
Krista Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01N 33/537
C12Q 1/48
G01N 33/53
G01N 33/543

US Classification:

435007920, 435015000

Abstract:

Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.

US Patent:

20090068694, Mar 12, 2009

Filed:

Sep 29, 2008

Appl. No.:

12/240823

Inventors:

Robert Zuk - Atherton CA, US
Sae Choo - San Jose CA, US
Weilei Ma - Palo Alto CA, US
Krista Witte - Hayward CA, US

Assignee:

FORTEBIO, INC. - Menlo Park CA

International Classification:

C12Q 1/48

US Classification:

435 15

Abstract:

Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.

US Patent:

20110236911, Sep 29, 2011

Filed:

Mar 18, 2011

Appl. No.:

13/052001

Inventors:

Sae Hyun Choo - Sunnyvale CA, US
Weilei Ma - Mountain View CA, US
Jing Wei - Sunnyvale CA, US

Assignee:

FORTEBIO, INC. - Menlo Park CA

International Classification:

G01N 33/53
C12M 1/34

US Classification:

435 791, 4352872

Abstract:

Improved apparatus, compositions, and methods for carrying out interferometry-based assays for detecting analytes in a sample through the use of a precipitating substrate to enhance an interferometry binding signal, and kits useful for carrying out these assays. Methods comprise providing an optical assembly comprising an optical element with a transparent material and adapted for coupling to a light source via a fiber, a first reflective surface and a second reflecting surface having a first analyte-binding molecule and separated from said first surface by a distance, d, exposing said optical element to a sample comprising said analyte, a second analyte binding molecule, and a precipitating substrate; and detecting a change in thickness at said first reflective surface thereby detecting said analyte in said sample. Kits include reagents for derivatizing assay components along with packaging and instructions for use.