Thomas Allen Neumann

US Patent:

7622298, Nov 24, 2009

Filed:

Mar 24, 2006

Appl. No.:

11/388920

Inventors:

Thomas Neumann - Seattle WA, US

Assignee:

Norits, Inc. - Gig Harbor WA

International Classification:

C12N 5/06

US Classification:

435395, 435325, 435397, 435 11, 435 12, 623 11, 623 141

Abstract:

Microvessel networks are produced in vitro from tissue-engineered parent vessels sprouting into a supporting matrix, as for example gels, of extracellular matrix proteins. The microvessel systems are integrated into devices that allow for controlled perfusion with fluids. The vessels may include cells from one cell type, for example, endothelial cells, or from combinations of two or more cell types.

US Patent:

7622299, Nov 24, 2009

Filed:

Feb 24, 2003

Appl. No.:

10/374755

Inventors:

Joan E. Sanders - Kirkland WA, US
Thomas Neumann - Seattle WA, US

Assignee:

University of Washington - Seattle WA

International Classification:

C12N 5/06

US Classification:

435398, 435395, 435399, 435402, 435325, 623 11, 623 115, 623 122, 623 127, 623 21, 623 2369, 623 237, 623 2372, 623 2376

Abstract:

In one aspect, the invention provides methods for forming a target tissue substitute. The methods of the invention comprise the following steps: (a) providing a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is designed to mimic the configuration of one or more structural elements in a target tissue; and (b) culturing cells on the scaffold to form a target tissue substitute. In another aspect, the invention provides implantable medical devices. The implantable medical devices of the invention comprise a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is arranged to mimic the configuration of one or more structural elements in a target tissue. Typically, cells are cultured on the scaffold to form a target tissue substitute.

US Patent:

7907765, Mar 15, 2011

Filed:

Sep 18, 2006

Appl. No.:

11/532648

Inventors:

Mark E. Fauver - Seattle WA, US
Eric J. Seibel - Seattle WA, US
Michael G. Meyer - Seattle WA, US
Alan C. Nelson - Gig Harbor WA, US
J. Richard Rahn - Sammamish WA, US
Thomas Neumann - Seattle WA, US
Roger H. Johnson - Phoenix AZ, US

Assignee:

University of Washington - Seattle WA
Visiongate, Inc. - Pheonix AZ

International Classification:

G06K 9/00

US Classification:

382131, 382132

Abstract:

An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

US Patent:

8003388, Aug 23, 2011

Filed:

Sep 24, 2007

Appl. No.:

11/860471

Inventors:

Thomas Neumann - Seattle WA, US

Assignee:

Nortis, Inc. - Gig Harbor WA

International Classification:

C12N 5/00

US Classification:

435395, 435325, 435397

Abstract:

A method for creating networks of perfusable microvessels in vitro. A mandrel is drawn through a matrix to form a channel through the matrix. Cells are injected into the channel. The matrix is incubated to allow the cells to attach inside the channel. The channel is perfused to remove unattached cells to create a parent vessel, where the parent vessel includes a perfusable hollow channel lined with cells in the matrix. The parent vessel is induced to create sprouts into the surrounding matrix gel so as to form a microvessel network. The microvessel network is subjected to luminal perfusion through the parent vessel.

US Patent:

8445280, May 21, 2013

Filed:

Sep 24, 2008

Appl. No.:

12/679429

Inventors:

Thomas Neumann - Seattle WA, US
Anna Tourovskaia - Mountlake Terrace WA, US
Mark E. Fauver - Seattle WA, US
Julia Oi Yan Yu - Kirkland WA, US

Assignee:

Nortis, Inc. - Seattle WA

International Classification:

C12N 5/07

US Classification:

435395, 435325, 435397

Abstract:

A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded into a channel in a matrix at to activate competency of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix. The sprouting parent vessels are grown until network forms.

US Patent:

20090317787, Dec 24, 2009

Filed:

Aug 14, 2009

Appl. No.:

12/541707

Inventors:

Thomas Neumann - Seattle WA, US

Assignee:

NORTIS, INC. - Gig Harbor WA

International Classification:

A01N 1/02
C12N 5/08

US Classification:

435 12, 435366

Abstract:

Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.

US Patent:

20120105600, May 3, 2012

Filed:

Jun 8, 2009

Appl. No.:

12/999515

Inventors:

Michael G. Meyer - Phoenix AZ, US
J. Richard Rahn - Sammamish WA, US
Anna V. Tourovskaia - Mountlake Terrace WA, US
Julia Oi Yan Yu - Santa Clara CA, US
Christy A. Lancaster - Seattle WA, US
Thomas Neumann - Seattle WA, US
Mark E. Fauver - Seattle WA, US

International Classification:

H04N 13/02

US Classification:

348 50, 348E13074

Abstract:

A method for 3D imaging of a biologic object () in an optical tomography system where a subcellular structure of a biological object () is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object () is moved relatively to a microscope objective () to present varying angles of view and the labeled biological object () is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object () and the microscope objective () within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object () from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ().