Gabriele Neumann

US Patent:

7968101, Jun 28, 2011

Filed:

Nov 18, 2005

Appl. No.:

11/283498

Inventors:

Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

A01N 63/00
A61K 39/00
A61K 39/12
A61K 39/145
A61K 45/00
C12Q 1/70
C12P 1/00
C12P 19/34
C12N 7/02
C12N 15/00
C12N 15/74
A61K 39/285

US Classification:

4242061, 4241841, 4242041, 4242051, 4242091, 424 936, 424 932, 4242811, 435 5, 435 41, 435 9133, 435239, 4353201, 435476, 435489

Abstract:

The invention provides a composition useful to prepare influenza viruses, e. g. , in the absence of helper virus, using vectors which include tandem transcription cassettes containing PolI and/or PolII promoters.

US Patent:

8163523, Apr 24, 2012

Filed:

Jun 9, 2009

Appl. No.:

12/481402

Inventors:

Pamuk Bilsel - Madison WI, US
Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Madison WI, US

Assignee:

Flugen, Inc. - Madison WI

International Classification:

C12P 19/34

US Classification:

435 911, 435358, 435325, 4242091

Abstract:

The present invention relates to a cell-based method for producing influenza virus vaccines by enriching the population of surface-bound α2,6-sialic acid receptors on a cell surface, such as on a Chinese Hamster Ovary (CHO) cell surface. The host cell therefore presents numerous binding sites to which an influenza virus can bind via its hemagglutinin spike protein and infect the host cell. In contrast to wild-type CHO cells, the surface of the mutated CHO cells of the present invention contains an enriched population of α2,6-sialic acid receptors which makes the inventive CHO cells highly susceptible to viral infection, and therefore safe, effective, and highly efficient cells for rapidly producing influenza vaccines.

US Patent:

20030035814, Feb 20, 2003

Filed:

Oct 4, 2001

Appl. No.:

09/971372

Inventors:

Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Nanuet NY, US

International Classification:

A61K039/21
C12N007/00

US Classification:

424/208100, 435/235100

Abstract:

The invention provides a composition useful to prepare influenza A viruses, e.g., in the absence of helper virus.

US Patent:

20030215794, Nov 20, 2003

Filed:

Jan 29, 2003

Appl. No.:

10/353856

Inventors:

Yoshihiro Kawaoka - Madison WI, US
Luke Jasenosky - Madison WI, US
Gabriele Neumann - Madison WI, US

International Classification:

C12Q001/70
C12N007/00
C12N007/02
C12N001/18
C12N005/06
C07K014/01

US Classification:

435/005000, 435/235100, 435/239000, 435/325000, 435/348000, 435/254200, 530/395000, 435/456000

Abstract:

Cloned filovirus genomic cDNA and methods of using the cDNA are provided. Further provided are noninfectious lipid encapsulated filovirus-based particles.

US Patent:

20060057116, Mar 16, 2006

Filed:

Jun 10, 2005

Appl. No.:

11/150631

Inventors:

Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Nanuet NY, US

International Classification:

A61K 48/00
C12N 5/08
C12N 15/86

US Classification:

424093200, 435456000, 435366000

Abstract:

The invention provides a composition useful to prepare influenza A viruses, e.g., in the absence of helper virus.

US Patent:

20060134138, Jun 22, 2006

Filed:

Apr 20, 2005

Appl. No.:

11/110117

Inventors:

Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Nanuet NY, US

International Classification:

A61K 39/145
C12N 5/06
C12N 15/86

US Classification:

424209100, 435456000, 435325000, 435349000

Abstract:

The invention provides a composition useful to prepare influenza A viruses, e.g., in the absence of helper virus.

US Patent:

20090017444, Jan 15, 2009

Filed:

Jun 7, 2007

Appl. No.:

11/810956

Inventors:

Yoshihiro Kawaoka - Middleton WI, US
Gabriele Neumann - Madison WI, US

Assignee:

WISCONSIN ALUMNI RESEARCH FOUNDATION - MADISON WI

International Classification:

C12Q 1/70

US Classification:

435 5

Abstract:

The invention provides methods to identify modulators of viral transcription or replication.

US Patent:

20090246830, Oct 1, 2009

Filed:

Jan 7, 2009

Appl. No.:

12/349769

Inventors:

Yoshihiro KAWAOKA - Middleton WI, US
Gabriele Neumann - Nanuet NY, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12P 21/02

US Classification:

435 693

Abstract:

A method for producing influenza virus particles for preparation of a vaccine. The method comprises growing reassortant influenza viruses to produce virus particles and recovering the produced virus particles. The reassortant influenza viruses are produced in cultured cells in the absence of helper virus by introducing into the cultured cells expression vectors sufficient to produce genomic vRNA or antigenomic vRNA (cRNA) segments in the cells of reassortant influenza viruses. The method produces a nucleoprotein and an RNA dependent RNA polymerase in the cells so that RNP complexes containing the genomic vRNA segments of the reassortant influenza viruses are formed. The method uses expression vectors containing HA, NA, non-HA and non-NA vRNA segments in which the HA and NA vRNA segments are HA and NA vRNA segments of a viral strain different from a strain that contains all of the non-HA and non-NA vRNA segments.