Siliang Living Zhou

US Patent:

8003407, Aug 23, 2011

Filed:

Jul 29, 2005

Appl. No.:

11/572568

Inventors:

Siliang Zhou - Hayward CA, US
William Rutter - San Francisco CA, US
Ning Liu - Sichuan, CN

Assignee:

Relia Diagnostic Systems, LLC - San Francisco CA

International Classification:

G01N 33/558

US Classification:

436514, 422401, 422420, 422421, 422422, 422424, 422425, 422426, 422427, 422429, 4352872, 4352877, 4352879, 435805, 435810, 435970, 436169, 436518, 436807, 436810

Abstract:

The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, such as samples containing cells and fluid. In general, according to the present invention, a test strip for a lateral flow assay for detection of at least one analyte in a sample comprises: (1) a chromatographic strip, a sample filter, a fluid-impermeable barrier, and means for providing a mobilizable detectable agent that is capable of binding to the at least one analyte or to the capture agent after capturing the analyte to the chromatographic strip such that the mobilizable detectable agent migrates through the chromatographic strip and contacts sample that has passed through the sample filter and also has migrated through the chromatographic strip. The test strip allows detection with or without quantitation of an analyte in a sample containing whole cells.

US Patent:

20030032196, Feb 13, 2003

Filed:

Jul 18, 2002

Appl. No.:

10/200058

Inventors:

Siliang Zhou - Hayward CA, US

International Classification:

G01N021/77
G01N001/18

US Classification:

436/169000, 436/170000, 436/177000, 436/178000, 422/056000, 422/058000, 422/060000, 422/101000

Abstract:

A test strip for conducting a lateral flow assay for detection of an analyte in a sample that contains cells and fluid is provided, as well as methods for making and using the test strip. This test strip is commercially usable, for example, in a diagnostic device at a point of care for detection of analytes in whole blood.

US Patent:

20100092945, Apr 15, 2010

Filed:

Oct 16, 2009

Appl. No.:

12/580981

Inventors:

Siliang Zhou - Hayward CA, US

Assignee:

RELIA DIAGNOSTIC SYSTEMS, LLC - San Francisco CA

International Classification:

C12Q 1/70
G01N 33/53

US Classification:

435 5, 435 71

Abstract:

A test strip for conducting a lateral flow assay for detection of an analyte in a sample that contains cells and fluid is provided, as well as methods for making and using the test strip. This test strip is commercially usable, for example, in a diagnostic device at a point of care for detection of analytes in whole blood.

US Patent:

20100099112, Apr 22, 2010

Filed:

Jun 2, 2005

Appl. No.:

11/569870

Inventors:

Siliang Zhou - Hayward CA, US
William J. Rutter - San Francisco CA, US
Ning Liu - Sichuan, CN

International Classification:

G01N 33/53
C12M 1/34

US Classification:

435 71, 4352878

Abstract:

The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, such as samples containing cells and fluid, where the assay is volume independent, and the sample size is less than about 100 μl, where the test strip includes a first membrane such as a sample filter, that is in capillary contact with an optional second membrane, such as a fluid collector, the second membrane, if present is in capillary contact with an optional third membrane, such as a conjugate pad containing a mobilizable detectable agent, or with a fourth membrane, which is a chromatographic strip, which optionally contains a mobilizable detectable agent, all such membranes being in fluid contact with a fifth membrane, such as a buffer pad, a sixth membrane, such as an absorbent pad, optionally a seventh membrane, which is also an absorbent pad, a capture band for capturing the analyte and at least one control band, or alternatively, the chromatographic strip contains the mobilizable detectable agent in place of a conjugate pad, where the test strip is configured to support removal of red blood cells from the sample and to allow uni-directional or bi-directional fluid flow of fluid from the sample filter to the capture band to be retained therein and detected thereon.

US Patent:

20120015429, Jan 19, 2012

Filed:

Jul 5, 2011

Appl. No.:

13/175215

Inventors:

Siliang Zhou - Hayward CA, US
William Rutter - San Francisco CA, US
Ning Liu - Chengdu, CN

Assignee:

RELIA DIAGNOSTIC SYSTEMS, INC - San Francisco CA

International Classification:

C12M 1/34
B01J 19/00

US Classification:

4352871, 422 681

Abstract:

The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, such as samples containing cells and fluid. In general, according to the present invention, a test strip for a lateral flow assay for detection of at least one analyte in a sample comprises: (1) a chromatographic strip, a sample filter, a fluid-impermeable barrier, and means for providing a mobilizable detectable agent that is capable of binding to the at least one analyte or to the capture agent after capturing the analyte to the chromatographic strip such that the mobilizable detectable agent migrates through the chromatographic strip and contacts sample that has passed through the sample filter and also has migrated through the chromatographic strip. The test strip allows detection with or without quantitation of an analyte in a sample containing whole cells.