Shaun P Healey

US Patent:

7547534, Jun 16, 2009

Filed:

Jun 16, 2003

Appl. No.:

10/517939

Inventors:

Brian Steer - San Diego CA, US
Walter Callen - San Diego CA, US
Shaun Healey - Carlsbad CA, US
Geoff Hazlewood - San Diego CA, US
Di Wu - San Diego CA, US
David Blum - San Diego CA, US
Alireza Esteghlalian - La Jolla CA, US

Assignee:

Verenium Corporation - San Diego CA

International Classification:

C12N 9/24
A23L 1/105
C12S 3/04
C12S 3/08

US Classification:

435190, 426 63, 435277, 435278

Abstract:

The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.

US Patent:

7960148, Jun 14, 2011

Filed:

Jul 2, 2004

Appl. No.:

10/560957

Inventors:

Brian Steer - San Diego CA, US
Walter Callen - San Diego CA, US
Shaun Healey - Carlsbad CA, US
Derrick Pulliam - Durham NC, US

Assignee:

Verenium Corporation - San Diego CA
Syngenta Participations AG - Basel

International Classification:

C12P 19/18
C12P 7/06
C12N 9/42
A61K 47/00

US Classification:

435 97, 435161, 435209, 424439

Abstract:

The invention relates to polypeptides having glucanase, e. g. , endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e. g. , endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e. g. , carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e. g. , endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.

US Patent:

8043839, Oct 25, 2011

Filed:

Feb 14, 2007

Appl. No.:

12/279326

Inventors:

David Weiner - Del Mar CA, US
David Blum - Nashville TN, US
Alexander Varvak - San Diego CA, US
Shaun Healey - Carlsbad CA, US
Kristine Chang - San Diego CA, US
Geoff Hazlewood - Newbury, GB
Thomas Todaro - San Diego CA, US
Grace Desantis - San Diego CA, US
Hwai Chang - San Marcos CA, US
Connie Jo Hansen - San Diego CA, US
Scott W. Beaver - San Marcos CA, US
Thomas Woodward - Scottsville VA, US
Charles Hancock - San Marcos CA, US

Assignee:

Verenium Corporation - San Diego CA

International Classification:

C12N 9/24
C12N 1/20
C12N 1/15
C12N 1/16
C12N 1/19
C12N 15/70
C12P 21/06
C07H 21/04
A01H 5/00

US Classification:

435200, 435 691, 4353201, 4352523, 536 232, 800298, 800312, 800314, 800320, 8003201, 8003202, 8003203

Abstract:

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e. g. , catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

US Patent:

20080248160, Oct 9, 2008

Filed:

Jun 16, 2003

Appl. No.:

10/463720

Inventors:

Brian Steer - San Diego CA, US
Walter Callen - San Diego CA, US
Shaun Healey - San Diego CA, US
Geoff Hazlewood - San Diego CA, US
Di Wu - San Diego CA, US
David Blum - San Diego CA, US
Alireza Esteghlalian - La Jolla CA, US

International Classification:

A21D 8/02
C12Q 1/68
C07H 21/04
C12P 21/06
C12N 9/24
C12N 15/74

US Classification:

426 20, 435 691, 435200, 435 6, 4352523, 435471, 536 232

Abstract:

The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.

US Patent:

20090038023, Feb 5, 2009

Filed:

Mar 10, 2006

Appl. No.:

11/817997

Inventors:

David P. Weiner - San Diego CA, US
Alexander Varvak - San Diego CA, US
Toby Richardson - San Diego CA, US
Mircea Podar - San Diego CA, US
Ellen Burke - San Diego CA, US
Shaun Healey - Carlsbad CA, US

Assignee:

Verenium Corporation - San Diego CA

International Classification:

C12N 9/88
C12N 15/60
C07H 21/00
C12P 19/34
C12N 15/63
C12N 1/21
C12N 11/02
C40B 40/10
G01N 33/573
A61K 9/14
A61K 9/127
A61K 38/45
C11D 3/386
C12N 15/87
A01H 1/00
C12Q 1/02
C12P 7/40
C40B 50/06
G06F 19/00
D21H 17/22
A23C 9/127
A23C 23/00
A23C 9/152
A23J 1/00
A23L 2/66
A21D 2/08
A21D 10/00
C07K 16/40
C12N 11/14
C12N 9/96
C12N 5/10
C12N 5/12
A01K 67/00
A01H 5/00

US Classification:

800 13, 536 232, 536 2353, 536 2433, 536 243, 435 912, 4353201, 4352523, 435348, 435419, 800298, 8003201, 8003203, 800312, 8003202, 800322, 800320, 435232, 435188, 435180, 435176, 435174, 506 18, 5303879, 435331, 435 74, 506 26, 435 29, 800278, 800292, 800294, 435136, 510392, 424 945, 435267, 4352625, 424450, 424489, 424422, 426 61, 426549, 426 7, 426590, 426656, 426580, 426582, 426583, 426 42, 162174, 702 22

Abstract:

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

US Patent:

20090221051, Sep 3, 2009

Filed:

May 7, 2009

Appl. No.:

12/402421

Inventors:

Brian STEER - San Diego CA, US
Walter Callen - San Diego CA, US
Shaun Healey - San Diego CA, US
Geoff Hazlewood - San Diego CA, US
Di Wu - San Diego CA, US
David Blum - San Diego CA, US
Alireza Esteghlalian - La Jolla CA, US

International Classification:

C12P 7/10
C12N 15/11
C12N 15/00
C12N 1/21
D21C 3/00

US Classification:

435165, 536 232, 4353201, 43525233, 435277

Abstract:

The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.

US Patent:

20110027346, Feb 3, 2011

Filed:

May 29, 2007

Appl. No.:

12/303088

Inventors:

David Weiner - Del Mar CA, US
Alexander Varvak - San Diego CA, US
Toby Richardson - San Diego CA, US
Mircea Podar - San Diego CA, US
Ellen Burke - San Diego CA, US
Shaun Healey - Carlsbad CA, US

Assignee:

VERENIUM CORPORATION - San Diego CA

International Classification:

A61K 9/127
C07H 21/04
C12N 15/63
C12N 1/21
C12N 5/10
C12N 1/15
C12N 1/19
A61P 7/00
C12N 9/88
C12N 11/16
C12N 11/14
C12N 11/08
C40B 40/10
C12P 7/40
A61K 38/51
A61K 9/14
A23C 9/12

US Classification:

424450, 536 232, 536 243, 4353201, 4352523, 435325, 43525411, 4352542, 435348, 435419, 435232, 435176, 435180, 506 18, 435136, 424 945, 424484, 435268, 426 42, 977906

Abstract:

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

US Patent:

20110117067, May 19, 2011

Filed:

Aug 4, 2007

Appl. No.:

12/375146

Inventors:

Alireza Esteghlalian - San Diego CA, US
Kenneth Barrett - Solana Beach CA, US
Shaun Healey - Carlsbad CA, US
Stacy M. Miles - Chapel Hill NC, US
Rene Quadt - Chapel Hill NC, US

International Classification:

A61K 35/12
C07H 21/04
C07H 21/00
C12N 15/63
C12N 5/10
C12N 1/21
C12N 1/15
C12N 1/19
A01H 5/10
A01H 5/00
A01H 1/00
A61K 38/47
C12P 1/00
C12P 19/00
C12P 7/04
C12P 7/06
C12P 7/16
A61P 43/00
C09K 8/02
C09K 8/60

US Classification:

424 9321, 536 231, 536 232, 4353201, 435325, 4352523, 43525231, 43525233, 43525234, 43525235, 43525411, 435419, 435348, 4352542, 800298, 8003201, 800320, 8003203, 8003202, 8003172, 8003174, 800312, 800306, 800322, 530324, 530350, 435 691, 435 912, 435 911, 435200, 800294, 424 9461, 424 932, 435267, 435 72, 435157, 435161, 435160, 435 41, 507101, 507201

Abstract:

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.