Skills:
Molecular biology cloning, qPCR, northern/western blotting, RNAi (shRNA and miRNA), ELISA, RIA Protein Chemistry purification and membrane protein by various column chromatography methods and characterization Cell biology culture of primary cells and embryonic stem cells, immunohistochemistry, confocal microscope, FACS, cell based transcription factor activity assay Virus lentivirus, retrovirus, adenovirus, adeno-associated virus, characterization and delivery of virus, and large scale production, diabetes-inducing EMC virus Synthesis of biomaterials chemical synthesis, polymerization, NMR, TEM Animal work mouse and rat, blood sampling, isolation organ tissue, systemic or portal vein injection and surgery (hepatectomy and spinal cord hemisection) Computational Biology Statistical program R, Ingenuity Pathway Analysis (IPA), Nonlinear dynamical systems (NLDS)