Ron S Broide

US Patent:

20050142589, Jun 30, 2005

Filed:

Oct 21, 2004

Appl. No.:

10/970760

Inventors:

Ron Broide - San Marcos CA, US
Alain Trembleau - Yerres, FR
Warren Young - San Diego CA, US
John Morrison - Rye NY, US
Floyd Bloom - San Diego CA, US

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

The present invention relates to methods and probes for detecting relative levels of multiple nucleic acid sequences of interest. The invention includes a method to detect the relative amounts of at least two target nucleic acid sequences in at least one sample by use of a corresponding set of detectably labelled oligonucleotide probes for each of the at least two target nucleic acid sequences, and detecting the hybridization of each of the corresponding sets of oligonucleotide probes to its respective target nucleic acid sequence. The invention also includes a method to detect the relative amounts of multiple target messenger RNAs in at least one microscopy sample by in situ hybridization using sets of oligonucleotide probes. Also disclosed are methods to provide corresponding sets of oligonucleotide probes for target nucleic acid sequences.

US Patent:

20210275691, Sep 9, 2021

Filed:

May 30, 2019

Appl. No.:

17/058037

Inventors:

- Irvine CA, US
Hsiang Ho - Irvine CA, US
Betsy Bloom - Anaheim CA, US
Greg Nicholson - Cypress CA, US
Lance Steward - Irvine CA, US
Terrence Hunt - Temecula CA, US
Ron Broide - San Marcos CA, US
David Rupp - San Pedro CA, US
David Canty - Irvine CA, US

International Classification:

A61K 49/00
G01N 33/50
A61K 9/00

Abstract:

An in vivo assay to evaluate topical delivery of a biologic agent from a formulation is provided. The assay comprises treating a skin area of a CD hairless rat with tape stripping to disrupt the stratum corneum in the skin area to define a treated skin area and topically applying a formulation to the treated skin area. The formulation, in one embodiment, comprises the biologic agent and a pharmaceutically acceptable carrier. Delivery of the biologic agent into the skin from the topically applied formulation is evaluated via in vitro or in vivo techniques.

US Patent:

20190343759, Nov 14, 2019

Filed:

May 10, 2019

Appl. No.:

16/408903

Inventors:

- Irvine CA, US
Amy Brideau-Andersen - San Clemente CA, US
Jie Shen - Irvine CA, US
Lydia Andrews-Jones - Lake Forest CA, US
Hsiang Ho - Irvine CA, US
Betsy Bloom - Anaheim CA, US
Guang Wei Lu - Irvine CA, US
Fangjing Wang - Irvine CA, US
Sunny Kumar - Aliso Viejo CA, US
Gregory Nicholson - Cypress CA, US
Ron Broide - San Marcos CA, US

International Classification:

A61K 9/00
A61Q 19/08
A61K 8/18
A61K 38/48

Abstract:

Methods for delivery of a biologic agent to the dermis or superficial muscles by topical application of the agent are provided. The methods comprise, in one embodiment, treating or conditioning a skin area to disrupt the stratum corneum in the skin area to define a treated skin area and applying a formulation comprising a biologic agent to the treated skin area.