Peter Y Tseng

US Patent:

20170362563, Dec 21, 2017

Filed:

Dec 15, 2015

Appl. No.:

15/536122

Inventors:

- Oakland CA, US
Coleman Murray - Los Angeles CA, US
Edward Pao - Diamond Bar CA, US
Peter Tseng - Saratoga CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12M 1/00
B03C 1/247
G01N 15/06
B03C 1/03
B03C 1/28
B03C 1/253
B03C 1/033
G01N 35/00
G01N 15/00

Abstract:

A system is provided for the quantitative magnetic separation of magnetic objects (e.g., particles or cells). The system uses magnetic ratcheting over arrays of ferromagnetic elements having gradient spacing manifested in various pitch zones that are encountered by the magnetic objects as they traverse the array. The system can be used to separate and concentrate magnetic objects based on iron oxide content. For cells, different phenotypes may be separated based, for example, on surface expression of proteins or molecules that are bound to magnetic particles. The system includes a substrate or chip having the array of ferromagnetic elements with increasing lateral pitch and an externally driven magnet device that generates a cycling magnetic field. Magnetic objects with higher IOC separate and equilibrate along the array at larger pitches. The system can be used for the differential sorting of particles and cells of interest.