Geoffrey Stuart Baird

US Patent:

6699687, Mar 2, 2004

Filed:

May 21, 1999

Appl. No.:

09/316920

Inventors:

Roger Y. Tsien - La Jolla CA
Geoffrey Baird - Solana Beach CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 1512

US Classification:

435 691, 4353201, 435325, 530350, 536 235

Abstract:

The present invention provides polypeptide and polynucleotides encoding fluorescent indicators having inserted within a fluorescent moiety a sensor polypeptide. Also provided are methods of using the fluorescent indicator. Circularly permuted fluorescent polypeptides and polynucleotides are also provided.

US Patent:

7005511, Feb 28, 2006

Filed:

Jul 29, 2002

Appl. No.:

10/209208

Inventors:

Roger Y. Tsien - LaJolla CA, US
Robert E. Campbell - San Diego CA, US
Geoffrey S. Baird - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07H 21/04

US Classification:

536 231, 4353201, 435 691, 435366, 435 96, 435 697, 530350

Abstract:

The present invention relates generally to variant fluorescent proteins, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. The invention also relates to methods of making and using such fluorescent protein monomers and dimers.

US Patent:

7022826, Apr 4, 2006

Filed:

Feb 26, 2001

Appl. No.:

09/794308

Inventors:

Roger Y. Tsien - La Jolla CA, US
David A. Zacharias - San Diego CA, US
Geoffrey S. Baird - Solana Beach CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07H 21/04
C12Q 1/68

US Classification:

536 231, 536 232, 536 234, 435 4, 435 6, 4353201, 435325, 435243, 435440

Abstract:

A non-oligomerizing fluorescent protein, which is derived from a fluorescent protein having at least one mutation that reduces or eliminates the ability of the fluorescent protein to oligomerize, is provided. The non-oligomerizing fluorescent protein can be derived from a naturally occurring green fluorescent protein, a red fluorescent protein, or other fluorescent protein, or a fluorescent protein related thereto. Also provided is a fusion protein, which includes a non-oligomerizing fluorescent protein linked to at least one polypeptide of interest. In addition, a polynucleotide encoding a non-oligomerizing fluorescent protein is provided, as is a recombinant nucleic acid molecule, which includes polynucleotide encoding a non-oligomerizing fluorescent protein operatively linked to at least a second polynucleotide. Vectors and host cells containing such polynucleotides also are provided, as are kits containing one or more non-oligomerizing fluorescent proteins or encoding polynucleotides or constructs derived therefrom. Further provided are methods of making and using the proteins and polynucleotides.

US Patent:

7060793, Jun 13, 2006

Filed:

Oct 23, 2001

Appl. No.:

09/999745

Inventors:

Roger Y. Tsien - La Jolla CA, US
Geoffrey Baird - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 14/00
C07K 1/00
C12N 15/00
C12N 15/12
C12N 15/63

US Classification:

530350, 530402, 435 697, 4353201, 435325

Abstract:

Polynucleotides encoding fluorescent indicators, which contain a sensor polypeptide inserted within a fluorescent moiety, are provided, as are polypeptides encoded by such polynucleotides. Also provided are circularly permuted fluorescent polypeptides and polynucleotides encoding the circularly permuted fluorescent polypeptides. In addition, methods of using the fluorescent indicators and the circularly permuted fluorescent polypeptides are provided.

US Patent:

7329735, Feb 12, 2008

Filed:

Jan 17, 2006

Appl. No.:

11/334218

Inventors:

Roger Y. Tsien - La Jolla CA, US
Robert E. Campbell - San Diego CA, US
Geoffrey S. Baird - San Diego CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A61K 49/00
C12N 15/09

US Classification:

530350, 530300, 435440

Abstract:

The present invention relates generally to variant fluorescent proteins, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. The invention also relates to methods of making and using such fluorescent protein monomers and dimers.

US Patent:

6469154, Oct 22, 2002

Filed:

May 21, 1999

Appl. No.:

09/316919

Inventors:

Roger Y. Tsien - La Jolla CA
Geoffrey Baird - Solana Beach CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 1563

US Classification:

536 235, 4353201, 435325, 435 691

Abstract:

The present invention provides polypeptide and polynucleotides encoding fluorescent indicators having inserted within a fluorescent moiety a sensor polypeptide. Also provided are methods of using the fluorescent indicator. Circularly permuted fluorescent polypeptides and polynucleotides are also provided.