Byounghoon B Hwang

Byounghoon Hwang Madison, WI

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Work:

UNIVERSITY OF WISCONSIN-MADISON

Jul 2014 to 2000
Associate Researcher University of Wisconsin-Madison
Madison, WI
Jun 2009 to Jul 2014
Postdoctoral research associate INDIANA UNIVERSITY
Indianapolis, IN
Aug 2003 to May 2009
Graduate Research Fellow in Ph.D. program INDIANA UNIVERSITY
Indianapolis, IN
Aug 2005 to Jul 2008
Teaching assistant Indiana University
Indianapolis, IN
Dec 2001 to Jul 2003
Research assistant NATIONAL CREATIVE RESEARCH INITIATIVE CENTER FOR GENOME REGULATION
Seoul, South Korea
Mar 2000 to Nov 2001
Research assistant DANKOOK UNIVERSITY
Seoul, South Korea
Mar 1998 to Feb 2000
Teaching assistant Dankook University
Seoul, South Korea
Mar 1998 to Feb 2000
Graduate Research Fellow in M.S. program

Education:

INDIANA UNIVERSITY
Indianapolis, IN
2003 to 2009
Ph.D. in Biochemistry and Molecular Biology DANKOOK UNIVERSITY
Seoul, South Korea
1998 to 2000
M.S. in Zoology (Molecular Biology) DANKOOK UNIVERSITY
Seoul, South Korea
1993 to 1998
B.S. in Molecular Biology

Skills:

* Molecular Biology: DNA & RNA manipulation, molecular cloning, mutagenesis, DNA sequencing, PCR, reverse transcription, quantitative real time PCR, Northern blotting, Western blotting, SELEX (systematic evolution of ligands by exponential enrichment), gel shift assay (EMSA), ELISA, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), GST or His pull-down assay. *Biochemistry: Purification of recombinant and endogenous proteins (from molecular cloning design to purification), Chromatography (GE AKTA FPLC systems), in vitro translation, in vitro transcription assay, in vitro kinase assay, in vitro helicase assay, in vitro NTPase assay, in vitro sumoylation assay. *Animal study: GTT assay, ITT assay, Isolation of hepatocyte from mouse, Isolation of mitochondria and measurement of respiratory rate, Measurement of enzyme activity in tissues and isolated primary cells: pyruvate dehydrogenase complex and citrate synthase, genotyping, DEXA (dual-energy X-ray absorptiometry), and analysis of metabolites in serum and tissues (Biochemical Journal 2009 and FEBS journal 2012). * Cell Biology: Knock-down experiment with siRNA, establishment of stable cell lines (knock-in and knock-down with shRNA, immunofluorescence analysis, yeast two-hybrid assay, luciferase assay, and nuclear import assay.