Won Kyu Lee

US Patent:

8304245, Nov 6, 2012

Filed:

Nov 2, 2007

Appl. No.:

11/934718

Inventors:

Franciscus Albertus Kuypers - El Cerritos CA, US
Won Chul Lee - Berkeley CA, US
Albert P. Pisano - Danville CA, US

Assignee:

Children's Hospital and Research Center at Oakland - Oakland CA

International Classification:

C12Q 1/34
C12Q 1/68
C12Q 1/00
C12M 1/34
C12M 1/00

US Classification:

436 63, 435 61, 435 4, 435 34, 4352887, 4352831

Abstract:

The invention provides a device, system and method that enables a microfluidic flow lysometer cell analyzer. Using a population of suspended living cells. cell surface molecule detection reagents, and cell cytoplasm (or nuclear) molecule detection reagents, this microfluidic cell analyzer can rapidly analyze a population of cells by running them on a one-at-a-time basis through small capillary channels. The cell's morphology or surface markers are analyzed, then the cells are lysed, and the molecules present in the cell's cytoplasm or nuclear material are analyzed. Cell morphology is then analyzed as the cell surface molecules are correlated with the molecules present in the same cell's cytoplasm or nucleic acids, and this correlated cell population data is then presented to a user for interpretation. The invention also addresses issues such as device fouling, correction for experimental artifacts (incomplete cell lyis, variable cell debris volume effects), and inadequate data collection that hampered earlier efforts in this area.

US Patent:

20110256183, Oct 20, 2011

Filed:

Mar 11, 2011

Appl. No.:

13/065030

Inventors:

Curtis W. Frank - Cupertino CA, US
Won Jae Lee - Mountain View CA, US
Nam-Joon Cho - Stanford CA, US
Jeffrey S. Glenn - Palo Alto CA, US

International Classification:

A61K 9/00
A61K 35/407
A61P 1/16
C12N 5/071
A61K 35/12
B82Y 5/00

US Classification:

424400, 435395, 424 937, 977773, 977923, 977915

Abstract:

Compositions and methods are provided for the manufacture and use of hydrogels with increased permeability to macromolecules with minimum loss of matrix mechanical strength and prepolymer viscosity for patternability. The hydrogels of the invention are formed from a prepolymer, which is polymerized in the presence of hydrophobic nanoparticles. In some embodiments of the invention cells are present during polymerization, and are encapsulated by the hydrogel. A high interfacial energy between the hydrophobic substrate and the aqueous polymerizing solution disrupts the hydrogel network structure, leading to network defects that increase permeability without loss of patternability.

US Patent:

20120065082, Mar 15, 2012

Filed:

Sep 1, 2011

Appl. No.:

13/224064

Inventors:

Franciscus Albertus Kuypers - El Cerritos CA, US
Won Chul Lee - Berkeley CA, US
Albert P. Pisano - Walnut Creek CA, US

Assignee:

Children's Hospital & Research Center at Oakland - Oakland CA

International Classification:

C40B 30/00
C40B 60/12

US Classification:

506 7, 506 39

Abstract:

An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.

Author:

Won Sul Lee

ISBN #:

0891071636

Author:

Won W. Lee

ISBN #:

0802809928

Author:

Won R Lee

ISBN #:

0893030082