Juan G Santiago

US Patent:

20130008789, Jan 10, 2013

Filed:

Sep 14, 2012

Appl. No.:

13/618228

Inventors:

Mostafa Ronaghi - San Diego CA, US
Tarun Khurana - Stanford CA, US
Juan G. Santiago - Stanford CA, US

Assignee:

The Board of Trustree of the Leland Stanford Junior University - Palo Alto CA

International Classification:

B03C 7/02
B81B 1/00

US Classification:

204451, 204643

Abstract:

Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like.