2010 to 2000 Post-doctoral FellowUniversity of Nevada School of Medicine
2009 to 2010Kansas State University College of Veterinary Medicine
2005 to 2009 Research Assistant ProfessorKansas State University College of Veterinary Medicine
2003 to 2005 Post-doctoral FellowState Key Lab of Reproductive Biology
2000 to 2003 Research AssistantShandong Agricultural University
1998 to 2000 Associate ProfessorXiaoxie, Shandong Province, China
1997 to 2000 Agricultural Scientific ConsultantShandong Agricultural University
1996 to 2000 Associate HeadCollege of Animal Science and Veterinary Medicine
1996 to 2000 DirectorTheriogenology
1996 to 2000Theriogenology
1995 to 2000Theriogenology
1988 to 2000Shandong Agricultural University
1992 to 1998Shandong Agricultural University
1988 to 1992 Assistant professor
Education:
Chinese Academy of Sciences 2000 to 2003 PhD in PhysiologyGansu Agricultural University College of Veterinary Medicine 1985 to 1988 MS in Reproductive EndocrinologyShandong Agricultural University College of Veterinary Medicine Tai'an, CN 1981 to 1985 DVM
2010 to 2000 Post-doctoral FellowUniversity of Nevada School of Medicine
2009 to 2010 Postdoctoral ScholarKansas State University College of Veterinary Medicine
2005 to 2009 Research Assistant ProfessorKansas State University College of Veterinary Medicine
2003 to 2005 Post-doctoral FellowState Key Lab of Reproductive Biology
2000 to 2003 Research AssistantShandong Agricultural University
1998 to 2000 Associate ProfessorTheriogenology
1996 to 2000 DirectorShandong Agricultural University
1992 to 1998 LecturerShandong Agricultural University
1988 to 1992 Assistant professor
Education:
Chinese Academy of Sciences 2000 to 2003 D.Sc. in PhysiologyGansu Agricultural University College of Veterinary Medicine 1985 to 1988 M.Sc. in EndocrinologyShandong Agricultural University College of Veterinary Medicine 1981 to 1985 B.V.Sc.
Skills:
DNA, RNA isolation and quality control Microarray; PCR; RT-PCR; qRT-PCR cDNA & siRNA or shRNA design and transfection Southern blotting, Northern blotting analyses and DNA sequencing Molecular cloning and subcloning; Generating Knock-out, Knock-in and mutation DNA constructs Biotinylation; immunoprecipitation (IP); Western blotting (SDS-PAGE, NuPAGE, TGX) Immunostaining and Confocal micrscopy, Flow cytometry Radioimmunoassay (RIA); Enzyme linked immunosorbent assay (ELISA) In vitro maturation of oocytes (IVM)/ In vitro culture of embryos (IVC) In vitro fertilization (IVF)/Intracytoplasmic sperm injection (ICSI) Embryo transfer and soft tissue surgery for animals ES cell line establishment and ES cell isolation Cryopreservation of sperm and early embryos Patch-clamping technique