Yangming M Wang

US Patent:

20120134966, May 31, 2012

Filed:

Apr 1, 2010

Appl. No.:

13/260904

Inventors:

Robert Blelloch - San Francisco CA, US
Robert Laird Judson - San Francisco CA, US
Deepa Subramanyam - San Francisco CA, US
Collin Alfred Melton - San Francisco CA, US
Yangming Wang - San Francisco CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A61K 35/12
C12N 5/0735
A61K 31/7105
A61P 25/16
A61P 25/28
A61P 21/00
A61P 9/10
A61P 35/00
A61P 3/00
A61P 3/10
A61P 9/00
A61P 1/00
A61P 19/08
A61P 19/10
A61P 19/02
A61P 25/00
A61P 35/02
C12N 5/071

US Classification:

424 937, 435377, 435366, 435325, 514 44 A

Abstract:

The methods of the present application describe that introduction of physiologically relevant miRNAs can enhance or modulate somatic cell reprogramming, generating induced pluripotent stem cells (iPS cells). These miRNAs did not further enhance reprogramming in the presence of cMyc. Furthermore, unlike previously described methods of generating iPS cells, such as through the introduction of genetic elements using viruses, the methods of the present invention reduce the risk of activating oncogenes in the iPS cells. The methods of the invention generate iPS cells that can be free of genetic modifications and thus have greater potential for use as therapeutic agents than those generated by existing methods.