Wonchul Suh from Lewes, DE

Parallel Chromosomal Stacking Of Traits In Bacteria
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US Patent:

7252942, Aug 7, 2007
Filed:

Dec 12, 2003
Appl. No.:

10/734778
Inventors:

Pierre E. Rouviere - Wilmington DE, US
Wonchul Suh - Hockessin DE, US
Assignee:

E.I. du Pont de Nemours and Company - Wilmington DE
International Classification:

C12Q 1/68
US Classification:

435 6, 435471, 435472
Abstract:

The invention describes a method for the stacking of traits in a recombination proficient host using a phage transduction system. The method makes use of a nucleic acid integration cassette that has homology to a specific site on a host chromosome for the insertion of genetic elements and the stacking of traits. Repetition of the method results in the stacking of traits on a single genetic element.

Method To Increase Hydrophobic Compound Titer In A Recombinant Microorganism
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US Patent:

7256014, Aug 14, 2007
Filed:

Jul 27, 2005
Appl. No.:

11/190386
Inventors:

Anthony J. Kinney - Wilmington DE, US
Hao Ni - Newark DE, US
Pierre E. Rouviere - Wilmington DE, US
Wonchul Suh - Hockessin DE, US
Assignee:

E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:

C12P 1/00
C12N 1/00
US Classification:

435 41, 4352523, 43525411, 4352572, 4353201
Abstract:

Expression of at least one plant oleosin gene in a microbial cell engineered to produce hydrophobic/lipophilic compounds significantly increases the overall titer of the compound. The hydrophobic nature of the oleosin core is believed to increase the microbial host cell's internal storage capacity for any hydrophobic/lipophilic compound. The method is exemplified using a bacterial host cell engineered to produce significant amount of various carotenoids.

Mutations Affecting Plasmid Copy Number
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US Patent:

7291482, Nov 6, 2007
Filed:

Dec 12, 2003
Appl. No.:

10/735019
Inventors:

Qiong Cheng - Wilmington DE, US
Pierre E. Rouviere - Wilmington DE, US
Luan Tao - Claymont DE, US
Wonchul Suh - Hockessin DE, US
Assignee:

E.I. du Pont de Nemours and Company - Wilmington DE
International Classification:

C12P 21/02
US Classification:

435 691, 43525233
Abstract:

Mutations in chromosomal genes have been identified that affect plasmid copy number in plasmids that are anti-sense RNA regulated such as the pMB1-derived and p15A-derived plasmids.

Method For Producing An L-Tyrosine Over-Producing Bacterial Strain
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US Patent:

7700328, Apr 20, 2010
Filed:

Jun 7, 2006
Appl. No.:

11/448331
Inventors:

Anthony A. Gatenby - Wilmington DE, US
Ranjan Patnaik - Newark DE, US
Fateme Sima Sariaslani - Wilmington DE, US
Wonchul Suh - Hockessin DE, US
Tina K. Van Dyk - Wilmington DE, US
Assignee:

E.I. du Pont de Nemours and Company - Wilmington DE
International Classification:

C12P 13/22
C12P 21/06
C12P 19/34
C12N 15/87
C12N 15/74
C12N 15/00
US Classification:

435108, 435463, 43525233, 435471, 435488, 435477, 435 691, 435 911, 4353201
Abstract:

An enteric bacterial strain was engineered to over-produce L-tyrosine using a one-step method. The pheA-tyrA chromosomal region of the bacterial genome was replaced with an engineered chromosomal segment, resulting in inactivation of the pheA coding region and strong expression of the tyrA coding region, resulting in high levels of L-tyrosine production.

Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof
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US Patent:

8241878, Aug 14, 2012
Filed:

Nov 15, 2011
Appl. No.:

13/296944
Inventors:

Larry Cameron Anthony - Aston PA, US
Dennis Flint - Newark DE, US
Wonchul Suh - Hockessin DE, US
Rick W. Ye - Hockessin DE, US
Steven Cary Rothman - Wilmington DE, US
Jean-Francois Tomb - Wilmington DE, US
Assignee:

Butamax(TM) Advanced Biofuels LLC - Wilmington DE
International Classification:

C12P 7/58
US Classification:

435137, 4352542, 435160
Abstract:

Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.

Enhanced Dihydroxy-Acid Dehydratase Activity In Lactic Acid Bacteria
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US Patent:

8637281, Jan 28, 2014
Filed:

Sep 29, 2009
Appl. No.:

12/569168
Inventors:

Brian James Paul - Wilmington DE, US
Wonchul Suh - Hockessin DE, US
Assignee:

Butamax(TM) Advanced Biofuels LLC - Wilmington DE
International Classification:

C12P 7/16
C12N 9/00
C12N 1/20
C12N 15/00
C07H 21/04
US Classification:

435160, 435183, 4352523, 4353201, 536 231, 536 232
Abstract:

Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0. 1 μmol minmg. LAB cells with even higher activities of 0. 2 to 0. 6 μmol minmgof DHAD activity were obtained. These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.

Method For Chromosomal Engineering
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US Patent:

20040209370, Oct 21, 2004
Filed:

Dec 12, 2003
Appl. No.:

10/734936
Inventors:

Wonchul Suh - Hockessin DE, US
International Classification:

C12N015/74
C12N001/21
US Classification:

435/488000, 435/252300
Abstract:

A method is provided for the integration of foreign genetic elements into a bacterial chromosome without the need of a cloning step. The method relies on the presence of the -Red recombinase system in a recombination proficient bacterial host. At least two linear recombination elements or constructs are co-transformed into the recombination proficient host which are assembled in the correction orientation in the bacterial chromosome by homologous recombination. Selectable markers used for the selection of transformants are later excised by the action of a site-specific recombination.

Increasing Carotenoid Production In Bacteria Via Chromosomal Integration
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US Patent:

20040219629, Nov 4, 2004
Filed:

Dec 12, 2003
Appl. No.:

10/735442
Inventors:

Qiong Cheng - Wilmington DE, US
Pierre Rouviere - Wilmington DE, US
Wonchul Suh - Hockessin DE, US
International Classification:

C12P023/00
C12N001/21
US Classification:

435/067000, 435/252330
Abstract:

The present invention relates to carotenoid overproducing bacteria The genes of the isoprenoid pathway in the bacterial hosts of the invention have been engineered such that certain genes are either up-regulated or down regulated resulting in the production of carotenoid compounds at a higher level than is found in the un-modified host. Genes that may be up-regulated include the dxs, idi, ispB, lytB and ygbBP genes. Additionally it has been found that a partial disruption of the yjeR gene has the effect of enhancing carotenoid production.

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Location:

Hockessin, DE

Work:

R and D Leader and Senior Principal Investigator

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Wonchul Suh