William R Donovan

US Patent:

6482636, Nov 19, 2002

Filed:

Aug 12, 1999

Appl. No.:

09/373257

Inventors:

William P. Donovan - Manchester MO
James A. Baum - Webster Groves MO

Assignee:

Certis USA, L.L.C. - Columbia MD

International Classification:

C12N 121

US Classification:

43525231, 4353201, 4352523, 4352525

Abstract:

The present invention provides constructed (. ) strains that are toxic to mosquito larvae. The mosquito-toxic. strains have improved characteristics, such as increased production of mosquito-toxic proteins, reduced number of spores or increased variety of mosquito-toxic proteins, compared with the well-known, mosquito-toxic strain subspecies ( ). The present invention involves insertion of an antibiotic-resistance gene into the mosquito-toxin plasmid having a size of approximately 75 mDa such that the plasmid is âtaggedâ with antibiotic resistance. The advantage of the tagged mosquito-toxin plasmid is that it permits detection of the rare event in which the mosquito-toxin plasmid is transferred from the strain (the donor strain) into a non- strain (the recipient strain) that normally does not harbor the mosquito-toxin plasmid. Non- recipient cells may have improved characteristics such as reduced spore numbers or increased variety of mosquito-toxic proteins. The present invention also provides for a mechanism of inactivating the antibiotic tag so that.

US Patent:

7662940, Feb 16, 2010

Filed:

Aug 1, 2005

Appl. No.:

11/194345

Inventors:

James A. Baum - Webster Groves MO, US
William P. Donovan - Manchester MO, US
Brian B. Hauge - Wildwood MO, US
Thomas J. LaRosa - Fenton MO, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C07H 21/00
A01H 1/00
A01H 5/00

US Classification:

536 231, 4353201, 435410, 800295, 800278, 530350

Abstract:

Nucleic acid sequences and insecticidal proteins encoded from these sequences are provided. Insecticidal proteins encoded by large operons situated within the primary sequence of large extrachromosomal plasmids present in are disclosed. Methods for making transgenic plants resistant to insect infestation as a result of expression of one or more of these proteins is disclosed. strains exhibiting broad host range insecticidal specificity are also disclosed.

US Patent:

8410334, Apr 2, 2013

Filed:

Feb 19, 2008

Appl. No.:

12/033178

Inventors:

Edwards Allen - O'Fallon MO, US
William P. Donovan - Manchester MO, US
Gregory R. Heck - Crystal Lake Park MO, US
James K. Roberts - Chesterfield MO, US
Virginia Ursin - Pawcatuck CT, US
Yuanji Zhang - Weldon Spring MO, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01H 1/00
C12N 15/82
C12N 15/87
C07H 21/04

US Classification:

800279, 800285, 800286, 536 245

Abstract:

This invention provides a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest, recombinant DNA constructs for expression of at least one invertebrate miRNA in planta and methods of use thereof, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention.

US Patent:

8569583, Oct 29, 2013

Filed:

Jun 29, 2011

Appl. No.:

13/171519

Inventors:

Judith Donovan - Manchester MO, US
William Donovan - Manchester MO, US
James Engleman - Ephrata PA, US
Thomas Malvar - North Stonington CT, US
John Pitkin - Wildwood MO, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01H 5/00
A01H 5/10
C12N 15/82
C12N 15/32
C07K 14/325

US Classification:

800302, 536 2371, 514 45, 424 932, 435418, 4353201, 800279

Abstract:

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from and related strains. The proteins are isolated from culture supernatants of and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

US Patent:

20060191034, Aug 24, 2006

Filed:

Jul 6, 2004

Appl. No.:

10/563270

Inventors:

James Baum - Webster Groves MO, US
Judith Donovan - Manchester MO, US
William Donovan - Manchester MO, US
James Engleman - Ephrata PA, US
Karina Krasomil-Osterfeld - Ellisville MO, US
John Pitkin - Wildwood MO, US
James Roberts - Chesterfield MO, US

International Classification:

A01H 1/00
C07H 21/04
C12N 15/82
C07K 14/325
A01N 63/00

US Classification:

800279000, 530350000, 514012000, 536023700, 435419000, 435468000

Abstract:

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from and related strains. The proteins are isolated from culture supernatants of and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle () and Southern Corn Rootworm (). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

US Patent:

20080040827, Feb 14, 2008

Filed:

Dec 14, 2004

Appl. No.:

10/581763

Inventors:

Judith Donovan - Manchester MO, US
William Donovan - Manchester MO, US
James Engleman - Ephrata PA, US
Thomas Malvar - North Stonington CT, US
John Pitkin - Wildwood MO, US

International Classification:

A01H 5/00
A01H 1/00
A61K 38/00
C07H 21/04
C07K 14/325
C12N 15/00
C12N 15/82
C12Q 1/68

US Classification:

800302, 4353201, 435410, 435 6, 514 2, 530350, 536 2371, 800279

Abstract:

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from and related strains. The proteins are isolated from culture supernatants of and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

US Patent:

20130165498, Jun 27, 2013

Filed:

Jan 22, 2013

Appl. No.:

13/746500

Inventors:

Monsanto Technology LLC - St. Louis MO, US
William P. Donovan - Manchester MO, US
Gregory R. Heck - Crystal Lake Park MO, US
James K. Roberts - Chesterfield MO, US
Virginia Ursin - Pawcatuck CT, US
Yuanji Zhang - Weldon Spring MO, US

Assignee:

MONSANTO TECHNOLOGY LLC - St. Louis MO

International Classification:

C12N 15/113

US Classification:

514 44 A, 4353201

Abstract:

This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

US Patent:

20130212748, Aug 15, 2013

Filed:

Nov 21, 2012

Appl. No.:

13/682774

Inventors:

MONSANTO TECHNOLOGY LLC - , US
William P. Donovan - Manchester MO, US
James T. Engleman - Ephrata PA, US
Thomas M. Malvar - North Stonington CT, US
John W. Pitkin - Wildwood MO, US

Assignee:

MONSANTO TECHNOLOGY LLC - St. Louis MO

International Classification:

C07K 14/32
C12N 15/82

US Classification:

800302, 536 2371, 530350, 435418, 514 45, 435412, 435416, 435414, 435415, 435 691, 4353201

Abstract:

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from and related strains. The proteins are isolated from culture supernatants of and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.