Willem G Langenberg

US Patent:

54160103, May 16, 1995

Filed:

Jun 10, 1993

Appl. No.:

8/074902

Inventors:

Willem G. Langenberg - Martell NE
Lingyu Zhang - Lincoln NE

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 1500
A01H 500
A01H 510

US Classification:

4351723

Abstract:

A method is provided for introducing a foreign gene into a plant cell by means of an Olpidium zoospore vector having associated with it a reassembled nucleoprotein complex comprising the foreign gene and reassociated coat protein of a zoospore-transmissable virus. The plant cell is contacted with the zoospore under conditions suitable for transmission of the nucleoprotein complex into the cell. The method can be conducted under nonsterile conditions, is applicable to all nucleic acids regardless of size, and is useful for introducing foreign genes into cells of both monocots and dicots.