Wendy L Thomas

US Patent:

20090325259, Dec 31, 2009

Filed:

Jan 15, 2009

Appl. No.:

12/354693

Inventors:

Viola Vogel - Seattle WA, US
Wendy Thomas - Seattle WA, US
Manu Forero - Seattle WA, US
Evgeni Sokurenko - Seattle WA, US

Assignee:

UNIVERSITY OF WASHINGTON - Seattle WA

International Classification:

C12N 11/00
C07K 14/00
C07K 16/00
C07K 14/42

US Classification:

435174, 530402, 530395, 5303911, 530370

Abstract:

Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules.

US Patent:

20040067544, Apr 8, 2004

Filed:

Jun 27, 2003

Appl. No.:

10/607834

Inventors:

Viola Vogel - Seattle WA, US
Wendy Thomas - Seattle WA, US
Manu Forero - Seattle WA, US
Evgeni Sokurenko - Seattle WA, US

International Classification:

C12Q001/68
G01N033/554
G01N033/569

US Classification:

435/007320

Abstract:

Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules.

US Patent:

20180193457, Jul 12, 2018

Filed:

May 13, 2016

Appl. No.:

15/573748

Inventors:

- Seattle WA, US
Dagmara KISIELA - SEATTLE WA, US
Wendy Evelyn THOMAS - SEATTLE WA, US
Veronika L. TCHESNOKOVA - SEATTLE WA, US

Assignee:

UNIVERSITY OF WASHINGTON - SEATTLE WA

International Classification:

A61K 39/40
A61P 31/04
A61P 1/04
A61K 39/02
C07K 14/245
C07K 16/12

Abstract:

Methods and antibody compositions that displace mannose from the binding pocket of fimbrial adhesin FimH of enterobacteria, including uropathogenic , or , can be used to disrupt or prevent the attachment of a single layer of bacteria to a mannose-coated surface, or to disrupt or prevent the formation of a multilayer biofilm. The antibody compositions of the invention can thus be used in methods to inhibit, prevent, or reverse the colonization of a surface with enterobacteria that express the bacterial Type 1 fimbrial adhesin FimH, to inhibit or prevent infection of a cell by enterobacteria that express the bacterial Type 1 fimbrial adhesin FimH, such as, for example, uropathogenic , to treat a bacterial infection in subject in need thereof and to treat or prevent inflammatory bowel disease (IBD), among other uses.

Author:

Wendy A. Thomas

ISBN #:

0802042279

Author:

Wendy Thomas

ISBN #:

0771056826