Vinod D Mirchandani

US Patent:

7208278, Apr 24, 2007

Filed:

Nov 4, 2004

Appl. No.:

10/982619

Inventors:

Caifu Chen - Palo Alto CA, US
Kevin Hennessy - San Mateo CA, US
Kai Qin Lao - Pleasanton CA, US
Teodoro Paner - Dublin CA, US
Vinod Mirchandani - San Ramon CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 242

Abstract:

The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

US Patent:

20050279949, Dec 22, 2005

Filed:

Nov 4, 2004

Appl. No.:

10/981440

Inventors:

Mark Oldham - Los Gatos CA, US
Vinod Mirchandani - Oak Park CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N021/01
F21V009/16

US Classification:

250458100, 356244000

Abstract:

A system is provided that includes a light-emitting diode (LED); a temperature sensor in thermal contact with the LED and capable of measuring an operating temperature and generating an operating temperature signal; and a temperature regulating system capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal. A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.

US Patent:

20070099228, May 3, 2007

Filed:

Dec 12, 2006

Appl. No.:

11/609776

Inventors:

Caifu Chen - Palo Alto CA, US
Kevin Hennessy - San Mateo CA, US
Kai Lao - Pleasanton CA, US
Teodoro Paner - Dublin CA, US
Vinod Mirchandani - San Ramon CA, US

Assignee:

APPLERA CORPORATION - Foster City CA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536024300

Abstract:

The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

US Patent:

20070105212, May 10, 2007

Filed:

Dec 22, 2006

Appl. No.:

11/644410

Inventors:

Mark Oldham - Los Gatos CA, US
Vinod Mirchandani - San Ramon CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34

US Classification:

435288700, 435289100, 435303100

Abstract:

A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.

US Patent:

20070259356, Nov 8, 2007

Filed:

Dec 12, 2006

Appl. No.:

11/609767

Inventors:

Caifu Chen - Palo Alto CA, US
Kevin Hennessy - San Mateo CA, US
Kai Lao - Pleasanton CA, US
Teodoro Paner - Dublin CA, US
Vinod Mirchandani - San Ramon CA, US

Assignee:

APPLERA CORPORATION - Foster City CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

US Patent:

20080038163, Feb 14, 2008

Filed:

Jun 1, 2007

Appl. No.:

11/757286

Inventors:

Steven Boege - San Mateo CA, US
John Sheridan - Marblehead MA, US
Ali Aslam - Salem MA, US
Dennis Lehto - Santa Clara CA, US
Julio Focaracci - San Mateo CA, US
Alexander Dromaretsky - Davis CA, US
Thomas Au - Palo Alto CA, US
Mark Oldham - Los Gatos CA, US
Eric Henderson - Fremont CA, US
Vinod Mirchandani - San Ramon CA, US
Eric Nordman - Palo Alto CA, US
Marc Haberstroh - San Jose CA, US
Johannes Sluis - Redwood City CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

F28F 99/00
B01J 19/00

US Classification:

422188000, 422198000

Abstract:

A device for performing biological analysis may include at least one reaction chamber configured to receive at least one sample for biological analysis and a thermal system configured to modulate a temperature of the at least one reaction chamber to cycle a temperature of the at least one biological sample. The thermal system may include a cooling system configured to cool the at least one reaction chamber. The cooling system may include a cooling fluid source positioned distally from the at least one reaction chamber, the cooling fluid source being in flow communication with at least one conduit configured to flow cooling fluid from the cooling fluid source to at least one location in thermal communication with the at least one reaction chamber.

US Patent:

20080050781, Feb 28, 2008

Filed:

Jun 22, 2007

Appl. No.:

11/767327

Inventors:

Mark Oldham - Los Gatos CA, US
Eric Henderson - Fremont CA, US
Vinod Mirchandani - San Ramon CA, US
Eric Nordman - Palo Alto CA, US
Marc Haberstroh - San Jose CA, US
Johannes Sluis - Redwood City CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12P 19/34
B01J 19/00
C12M 1/38

US Classification:

435091200, 422105000, 435286100

Abstract:

A device for performing polymerase chain reactions using cooling members not used in conventional thermal cyclers. A device for performing polymerase chain reactions may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may comprise at least one cooling member selected from a synthetic jet ejector array, a vibration-induced droplet atomization system, a vibrating diaphragm system, a piezo fan, a Cold Gun, a microchannel cooling loop, and a Cool Chip.

US Patent:

20100015631, Jan 21, 2010

Filed:

Sep 14, 2009

Appl. No.:

12/559476

Inventors:

Caifu CHEN - Palo Alto CA, US
Kevin Hennessy - San Mateo CA, US
Kai Qin Lao - Pleasanton CA, US
Teodoro Paner - Dublin CA, US
Vinod Mirchandani - San Ramon CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 6, 536 231, 536 2433

Abstract:

The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.