Vanda J White

US Patent:

20130089886, Apr 11, 2013

Filed:

Oct 8, 2012

Appl. No.:

13/647072

Inventors:

William B. Brasso - Columbia MD, US
Song Shi - Reisterstown MD, US
Ben Turng - Ellicott City MD, US
Susan M. Kircher - Hanover PA, US
Vanda White - Baltimore MD, US
Dyan Luper - Cockeysville MD, US
Julie Rosales - Randallstown MD, US
Gretta Campbell - Glen Rock PA, US
Adrien Malick - Granite MD, US

Assignee:

BECTON, DICKINSON AND COMPANY - Franklin Lakes NJ

International Classification:

C12Q 1/24

US Classification:

435 30

Abstract:

Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of

US Patent:

20200087702, Mar 19, 2020

Filed:

Nov 7, 2019

Appl. No.:

16/677092

Inventors:

- Franklin Lakes NJ, US
Vanda White - Baltimore MD, US
William B. Brasso - Columbia MD, US
Dyan Luper - Cockeysville MD, US
James Y. Zhou - Columbia MD, US
Julie L. Rosales - Randallstown MD, US
Jeffery H. Bruton - Randallstown MD, US
John D. Mantlo - Sykesville MD, US
Adrien P. Malick - Granite MD, US
Donald R. Callihan - Cockeysville MD, US
Ben Turng - Tucson AZ, US
Liping Feng - Baltimore MD, US
Curtis M. Gosnell - Fallston MD, US
Patrick Shawn Beaty - Dallastown PA, US
John P. Douglass - York PA, US

Assignee:

BECTON DICKINSON AND COMPANY - Franklin Lakes NJ

International Classification:

C12Q 1/14
C12Q 1/24
C12N 1/02
C12N 1/20

Abstract:

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including , from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

US Patent:

20170204448, Jul 20, 2017

Filed:

Mar 29, 2017

Appl. No.:

15/472759

Inventors:

- Franklin Lakes NJ, US
William B. Brasso - Columbia MD, US
Susan M. Kircher - Hanover PA, US
Vanda White - Baltimore MD, US
Song Shi - Reisterstown MD, US
Xiao Mo - Ellicott City MD, US
Tuan-Linh Ngoc Nguyen - Carlisle PA, US
Adrien P. Malick - Sparks MD, US
Jon E. Salomon - Stewartstown PA, US
John D. Mantlo - Sykesville MD, US
Mary R. Votta - White Hall MD, US
Ben Turng - Ellicott City MD, US
Donald R. Callihan - Cockeysville MD, US
Wendy Louise Williams - Windsor PA, US

International Classification:

C12Q 1/04
G01N 33/483

Abstract:

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

US Patent:

20150125895, May 7, 2015

Filed:

Feb 28, 2013

Appl. No.:

14/381388

Inventors:

- Franklin Lakes NJ, US
Vanda White - Baltimore MD, US
William B. Brasso - Columbia MD, US
Dyan Luper - Cockeysville MD, US
James Y. Zhou - Columbia MD, US
Julie L. Rosales - Randallstown MD, US
Jeffery H. Bruton - Randallstown MD, US
John D. Mantlo - Westminster MD, US
Adrien P. Malick - Sparks MD, US
Donald R. Callihan - Cockeysville MD, US
Ben Turng - Tucson AZ, US
Liping Feng - Baltimore MD, US
Curtis M. Gosnell - Fallston MD, US
Patrick Shawn Beaty - Dallastown PA, US
John P. Douglass - York PA, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q 1/14
C12N 1/20
C12Q 1/24

US Classification:

435 30, 4352534

Abstract:

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including , from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.