Skills:
7 years working on infectious diseases and cancer gene expression for candidate evaluation & gene therapy. 6+ years non-viral based genetic modification of cell lines, primary cells and hematopoietic stem cells 8 years si/sh/microRNAs design, application and analysis of side effect/pathway perturbation. 9 years genetic modification of highly different bacterial organisms. 6+ years lentiviral vector de novo design/optimization/transducti... of cell lines, primary cells and hematopoietic stem cells (4+ years for bone marrow transplant based gene therapy, RNAi, plasmids, therapeutic proteins). 8+ years working with infectious pathogens (HIV, tuberculosis, Listeria) under varying safety requirements (BSL2, BLS2+ and BLS3). Broad spectrum of molecular biology techniques (including multi-color flow, ELISA, qRT-PCR etc). 6+ years analysis of lentiviral vector & HIV replication, transgene expression, therapeutic effect analysis in short & long term experiments. 6+ years virus production & purification (small and large scale for in vitro and in vivo experiments). 7 years extensive experience with RNA/DNA/protein sequence analysis software CLC Workbench (including genomic data/NGS), informatics experience (scripting in javascript & C-like PAWN). 9+ years designing complex genetic constructs (plasmid, transgene vectors, and synthesized nucleic acids) with techniques including Gibson assembly & sophisticated PCR technology (fusion PCR, step-up etc) Experience in budgeting for in vitro and in vivo preclinical experiments as part of an in-human pilot study, multi-year grant application. Training in design, statistics, application, and analysis of clinical studies. 9+ years supervising and training students (up to 2; recently my 1st Master student graduated). 6+ years inventory control & large equipment negotiation for 20+ research team. Excellent written & oral communication as well as presentation skills (both English & German), acting as author & reviewer for peer review