Tiffany A Freed

US Patent:

20190346455, Nov 14, 2019

Filed:

Dec 14, 2017

Appl. No.:

16/466451

Inventors:

- Morrisville NC, US
Tiffany A. FREED - Apex NC, US
Deirdre M. HAUSER - Hillsborough NC, US
Kelli GOODMAN - Raleigh NC, US

International Classification:

G01N 33/68
G01N 33/70
G01N 33/62
G01N 30/72
H01J 49/00
H01J 49/14

Abstract:

A method for determining in a sample, by mass spectrometry, the amount of one or more analytes selected from the group consisting of N-acetylthreonine, TMAP, phenylacetylglutamine, tryptophan, creatinine, meso-erythritol, arabitol, myo-inositol, N-acetyl serine, N-acetylalanine, 3-methylhistidine, trans-4-hydroxyproline, kynurenine, urea, C-glycosyltryptophan, 3-indoxyl sulfate, pseudouridine, and combinations thereof is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more of the analytes; measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount of the one or more ions to determine the amount of each of the one or more analytes in the sample. Also described is a kit comprising one or more isotopically labeled analogues as internal standards for each of the one or more analytes.