Susan Blank Slemenda

US Patent:

51069652, Apr 21, 1992

Filed:

Apr 9, 1990

Appl. No.:

7/507421

Inventors:

Norman J. Pieniazek - Suwanee GA
Susan B. Slemenda - Decatur GA
Danuta Pieniazek - Suwanee GA
Jorge Velarde - San Diego CA
Ronald B. Luftig - Metairie LA

Assignee:

Research Corporation Technologies, Inc. - Tucson AZ

International Classification:

C12Q 170
C07K 300

US Classification:

536 27

Abstract:

The present invention relates to DNA and proteins of human adenovirus Type 41 and their use in detection of said virus. More specifically, the present invention relates to the isolation of a 41. 4 kd short fiber protein and a 60. 6 kd long fiber protein of adenovirus type 41 (Ad41), as well as proteins derived from the Ad41 E3 region, thereby providing virus-derived antigens and active derivatives and parts thereof, useful in the development of diagnostic assays, DNA probes and vaccines for said virus or other related viruses belonging to the human enteric adenovirus family. In addition, the present invention is directed to recombinant DNA molecules containing the human enteric adenovirus Type 41 Tak long fiber protein gene, the Ad41 short fiber protein gene and the Ad41 E3 gene (encoding the Ad41 proteins RL-1 to RL-6) thereby providing a source of recombinant viral components useful in the development of said diagnostic assays, DNA probes and vaccines for human adenoviruses. The present invention is also directed to first antibodies specific to the above-identified Ad41 viral components and to second antibodies specific to the first antibodies. These second antibodies are also useful in the development of diagnostic assays for Ad41 and other adenoviruses.