Susan L Hobbs

Author:

Susan Hobbs

ISBN #:

0934686343

US Patent:

20030104451, Jun 5, 2003

Filed:

Oct 31, 2002

Appl. No.:

10/285810

Inventors:

Susan Hobbs - Fremont CA, US
Mark Bednarski - Los Altos CA, US
Yi-Shan Yang - Saratoga CA, US
Samira Guccione - Hillsborough CA, US
Gongyi Shi - Dublin CA, US

International Classification:

C12Q001/68
G01N033/53
C12M001/34

US Classification:

435/006000, 435/007100, 435/287200

Abstract:

Devices and methods of use and manufacture for the identification and characterization of analytes, e.g. proteins, are provided. The subject devices are characterized by having a substrate with a polymerized monomolecular film over at least a portion of the substrate, the monomolecular film having at least one ligand or specific binding pair member associated therewith. Preferably the monomolecular film is stable to the laser intensities employed in MALDI-MS. In certain embodiments, the ligands are biotin, integrin antagonists, antibodies and antigens. In using the subject devices, a subject device is contacted with a sample. If present in the sample, a member of the binding pair of interest binds to its complementary ligand and, once bound, can be analyze by mass spectroscopy techniques. Also provided are kits, which include the subject devices.