Steven M Menchen

US Patent:

6355709, Mar 12, 2002

Filed:

Oct 15, 1997

Appl. No.:

08/950926

Inventors:

Ramakrishna S. Madabhushi - Foster City CA
Steven M. Menchen - Fremont CA
J. William Efcavitch - San Mateo CA
Paul D. Grossman - Burlingame CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C08K 516

US Classification:

524104, 524210, 524211, 524216, 524555

Abstract:

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20Â C. to about 50Â C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5Ã10 to about 1Ã10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

US Patent:

6358385, Mar 19, 2002

Filed:

Jan 11, 1999

Appl. No.:

09/228991

Inventors:

Ramakrishna S. Madabhushi - Foster City CA
Steven M. Menchen - Fremont CA
J. William Efcavitch - San Mateo CA
Paul D. Grossman - Burlingame CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

B01D 5702

US Classification:

204451, 204450, 204453, 204601, 204603, 204454, 204452

Abstract:

The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20Â C. to about 50Â C. , (ii) concentration in a separation medium in the range between about 0. 001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5Ã10 to about 1Ã10 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9. In one embodiment, polymers of the invention are selected from the group consisting of polylactams, such as polyvinylpyrrolidone; N,N-disubstituted polyacrylamides; and N-substituted polyacrylamides. In accordance with the method of the invention, a sufficient amount of polymer adsorbs to the capillary surface to establish a zone of high viscosity that shields the analyte from the wall and impedes the movement of an electrical double layer under an electric field.

US Patent:

6403812, Jun 11, 2002

Filed:

May 30, 2000

Appl. No.:

09/580754

Inventors:

Steven M. Menchen - Fremont CA
Linda G. Lee - Palo Alto CA
Charles R. Connell - Redwood City CA
N. Davis Hershey - San Carlos CA
Vergine Chakerian - San Mateo CA
Sam L. Woo - Redwood City CA
Steven Fung - Palo Alto CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C07D31182

US Classification:

549223, 549224, 549225, 549382, 549388

Abstract:

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

US Patent:

6566071, May 20, 2003

Filed:

Oct 2, 2001

Appl. No.:

09/969430

Inventors:

Scott C. Benson - Alameda CA
Joe Y. L. Lam - Castro Valley CA
Steven Michael Menchen - Fremont CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6, 536 266, 549382, 548417

Abstract:

Dibenzorhodamine compounds having the structure: are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure: including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, âCH OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

US Patent:

6504024, Jan 7, 2003

Filed:

Jun 14, 2001

Appl. No.:

09/881898

Inventors:

Shaheer H. Khan - Foster City CA
Steven M. Menchen - Fremont CA
Barnett B. Rosenblum - San Jose CA

Assignee:

PE Corporation (NY) - Foster City CA

International Classification:

C07H 1900

US Classification:

536 2626, 536 263, 536 266, 536 267, 536 2674, 536 268, 536 271, 536 2713, 536 2721, 536 276, 536 278, 536 2781, 536 281, 536 283, 536 284, 536 2853, 536 2854, 435 6, 435 89, 435 90, 435 911, 435 9153

Abstract:

Propargylethoxyamino nucleosides are disclosed having the structure wherein R and R are âH, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W is âH or âOH; W is âOH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3â-position; and W is âPO , âP O , âP O , phosphate analog, or âOH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

US Patent:

6583168, Jun 24, 2003

Filed:

Nov 28, 2000

Appl. No.:

09/724855

Inventors:

Steven M. Menchen - Fremont CA
Scott C. Benson - Alameda CA
Joe Y. L. Lam - Castro Valley CA
Weiguo Zhen - Foster City CA
Daqing Sun - Foster City CA
Barnett B. Rosenblum - San Jose CA
Shaheer H. Khan - Foster City CA
Meng Taing - San Mateo CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07D31178

US Classification:

514410, 514453, 549381, 549382, 548417

Abstract:

Sulfonated diarylrhodamine compounds are useful as fluorescent labels of nucleosides, nucleotides, polynucleotides, and polypeptides. The compounds find particular application in the area of fluorescent nucleic acid analysis, e. g. , automated DNA sequencing and fragment analysis, detection of probe hybridization in hybridization arrays, detection of nucleic acid amplification products, and the like.

US Patent:

6617445, Sep 9, 2003

Filed:

Oct 11, 2001

Appl. No.:

09/976842

Inventors:

Scott C. Benson - Alameda CA
Steven M. Menchen - Fremont CA
Peter D. Theisen - Reno NV
Kevin M. Hennessey - San Mateo CA
Vergine C. Furniss - San Mateo CA
Joan Hauser - Oakland CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 1904

US Classification:

536 266, 536 2534, 549223, 549224, 544105

Abstract:

A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y and Y are individually hydroxyl, amino, imminium, or oxygen, R -R are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof and R is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure where substituents R -R correspond to like-referenced substituents in the structure of described above, and Y is hydroxyl or amine. In another aspect the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides.

US Patent:

6649598, Nov 18, 2003

Filed:

Sep 7, 2001

Appl. No.:

09/949444

Inventors:

Steven M. Menchen - Fremont CA
Linda G. Lee - Palo Alto CA
Charles R. Connell - Redwood City CA
N. Davis Hershey - San Carlos CA
Vergine Chakerian - San Mateo CA
Sam L. Woo - Redwood City CA
Steven Fung - Palo Alto CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

A01N 4304

US Classification:

514 44, 435 6, 435 91, 435968, 536 231, 536 243, 536 2532, 536 254, 536 256, 436 86, 436 94, 436 96, 436 98, 436161, 436162, 436800, 935 77

Abstract:

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.