Steve C Hobbs

US Patent:

20190002956, Jan 3, 2019

Filed:

Jun 28, 2018

Appl. No.:

16/022500

Inventors:

- Hercules CA, US
George CARMAN - Livermore CA, US
Steve HOBBS - Pleasanton CA, US
Dmitri SIMONIAN - Mountain View CA, US
David GLADE - San Ramon CA, US
Joshua OEN - Fremont CA, US
Denis PRISTINSKI - Pleasanton CA, US
John DZENITIS - Danville CA, US

International Classification:

C12Q 1/686
B01L 3/00
G01N 15/14
B01F 3/08
C12Q 1/6848
C12Q 1/6844

Abstract:

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

US Patent:

20180229239, Aug 16, 2018

Filed:

Feb 12, 2018

Appl. No.:

15/894836

Inventors:

- Hercules CA, US
John DZENITIS - Danville CA, US
Steve HOBBS - Pleasanton CA, US
Stefano SCHIAFFINO - Pleasanton CA, US

International Classification:

B01L 3/00
G01N 1/38
B01F 3/08
B01F 13/10

Abstract:

Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.