Stephen C Kowalczykowski

US Patent:

6852832, Feb 8, 2005

Filed:

Aug 4, 2000

Appl. No.:

09/631616

Inventors:

Stephen C. Kowalczykowski - Davis CA, US
Erica M. Seitz - Davis CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K014/195
C07H021/02
C07H021/04

US Classification:

530350, 530358, 536 237

Abstract:

The invention provides ssDNA-binding proteins from three species of archaeons, , and , as well as the ability to identify ssDNA-binding proteins from other archaeons. The proteins help render DNA more accessible to DNA polymerase and are robust reagents for a variety of biotechnical processes, including PCR. The invention further provides nucleic acids encoding such proteins, vectors for transfecting host cells, host cells comprising the vectors, and methods of using the proteins.

US Patent:

7666591, Feb 23, 2010

Filed:

Jan 14, 2005

Appl. No.:

11/035703

Inventors:

Stephen C. Kowalczykowski - Davis CA, US
Erica M. Seitz - Davis CA, US

Assignee:

The Regents of the University of California - Oakland CA
The United States of America as represented by the Department of Health and Human Services - Washington DC

International Classification:

C12Q 1/68
C12P 19/34
C07K 14/195
C07H 21/02
C07H 21/04

US Classification:

435 6, 435 912, 530350, 530358, 536 237

Abstract:

The invention provides ssDNA-binding proteins from three species of archaeons, , and , as well as the ability to identify ssDNA-binding proteins from other archaeons. The proteins help render DNA more accessible to DNA polymerase and are robust reagents for a variety of biotechnical processes, including PCR. The invention further provides nucleic acids encoding such proteins, vectors for transfecting host cells, host cells comprising the vectors, and methods of using the proteins.

US Patent:

20040180342, Sep 16, 2004

Filed:

Mar 11, 2003

Appl. No.:

10/386575

Inventors:

Cynthia Haseltine - Davis CA, US
Stephen Kowalczykowski - Davis CA, US

Assignee:

REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12Q001/68
C12P019/34
C12N009/22

US Classification:

435/006000, 435/091200, 435/199000

Abstract:

The invention provides multimers of ssDNA binding protein that bind single stranded DNA. The multimers are robust and stable reagents for use in PCR and other techniques for engineering DNA. The invention further provides methods for performing nucleic acid amplification and engineering using the multimers.

US Patent:

20110177501, Jul 21, 2011

Filed:

Jun 11, 2008

Appl. No.:

12/451954

Inventors:

Mark Dillingham - London, GB
Stephen C. Kowalczykowski - Davis CA, US
Martin Webb - London, GB

International Classification:

C12Q 1/68
C07K 14/245

US Classification:

435 611, 530350, 530402, 435 613

Abstract:

A modified protein of the single strand DNA-binding domain, SSB family comprising a detectable label is disclosed. The label has detectable characteristics which alter on binding single stranded DNA. The protein is thus useful in an assay for single stranded DNA.

US Patent:

20120058478, Mar 8, 2012

Filed:

Nov 8, 2010

Appl. No.:

12/941761

Inventors:

Cynthia A. Haseltine - Davis CA, US
Stephen C. Kowalczykowski - Davis CA, US

Assignee:

REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12Q 1/68
C12P 19/34
C07K 14/00

US Classification:

435 612, 530408, 435 912

Abstract:

The invention provides multimers of ssDNA binding protein that bind single stranded DNA. The multimers are robust and stable reagents for use in PCR and other techniques for engineering DNA. The invention further provides methods for performing nucleic acid amplification and engineering using the multimers.

US Patent:

20130203116, Aug 8, 2013

Filed:

Aug 5, 2011

Appl. No.:

13/814713

Inventors:

Stephen Kowalczykowski - Davis CA, US
Ryan Jensen - Davis CA, US

Assignee:

The Regents of the University of California 1111 Franklin Street, 12th Floor - Oakland CA

International Classification:

C12P 21/00

US Classification:

435 697, 530350, 4353201, 435369, 436501

Abstract:

The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BR-CA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described.

US Patent:

57472475, May 5, 1998

Filed:

Jul 25, 1994

Appl. No.:

8/280020

Inventors:

Stephen C. Kowalczykowski - Davis CA
Angela K. Eggleston - Davis CA

Assignee:

The Regents of The University of California - Oakland CA

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

The invention provides spectroscopic methods for detecting helicase activity and inhibitors of helicase activity. Samples are assayed for helicase activity by: (a) incubating a mixture of the sample, double-stranded nucleic acid and a suitable luminescent marker which lumineses selectively in the presence of double-stranded nucleic acid; (b) exposing the mixture to light capable of inducing luminescence from the marker; and (c) detecting the intensity of luminescence from the mixture. Alternatively, samples are assayed for helicase inhibitors by further including in the mixture a helicase and incubating the mixture under conditions whereby, but for the presence of an inhibitor of the helicase in the sample, the helicase would be capable of converting a portion of the double-stranded nucleic acid into single-stranded nucleic acid. In both assays, helicase activity is inversely proportional to the detected luminescense. The methods are particularly suited to high-throughput drug screening.