Christopher Yee - Needham MA Todd Blythe - Malden MA Spencer Shames - Concord MA Elizabeth Rogers - Cambridge MA
Assignee:
Genzyme Corporation - Cambridge MA
International Classification:
C12N 948 C12P 762 C12P 4100
US Classification:
435280
Abstract:
A method for the resolution of. alpha. -tertiary carboxylic acid esters by partial hydrolysis is disclosed. The partial hydrolysis is carried out by contacting the esters with an aqueous solution in the presence of a serine carboxypeptidase, and the hydrolysis product is separated from unreacted starting material to obtain the hydrolysis product or the unreacted starting material in enantiomerically enriched form. A novel ester hydrolase which is particularly useful in this method, and a nucleotide sequence encoding this enzyme, are also described.
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