Cleveland State University Aug 2008 - Jun 2013
Graduate Teaching Assistant
Cleveland State University Aug 2009 - May 2013
Graduate Research Assistant
Tryzub Feb 2003 - Jul 2006
Veterinary Technical Assistant
Greenwich Valley Veterinary Clinic Jun 1998 - Dec 2002
Veterinary Technical Assistant
Education:
Cleveland State University 2008 - 2013
Master of Science, Masters, Molecular Biology
Rhode Island College 1992 - 1998
Bachelors, Bachelor of Arts, Biology, Music
Skills:
Dna Ligation Dna Recombination Cell Biology Biology Bacterial Transformation Gel Electrophoresis Dna Electrophoresis Genomics Plasmid Isolation Vector Cloning Data Analysis Restriction Digestion Bacterial Culture Pcr Cell Dna Agarose Gel Electrophoresis Molecular Biology Streak Plating Blast Molecular Genetics Microbiology Molecular Cloning Research Genetics Dna Extraction Fluorescence Microscopy Microscopy Miniprep
Interests:
Japanese Culture Kung Fu Physical Fitness Home Theater
Aug 2008 to 2000 Graduate Teaching AssistantWal-Mart Incorporated North Olmsted, OH Jun 2007 to Aug 2008 Electronics Sales AssociateWal-Mart Incorporated Fall River, MA Sep 2006 to Jun 2007 Electronics Sales AssociateTryzub Inc Cleveland, OH Feb 2003 to Jul 2006 dba West Park Animal HospitalGreenwich Valley Veterinary Clinic West Warwick, RI Jun 1998 to Dec 2002 Veterinary Technical Assistant
Education:
Cleveland State University Cleveland, OH May 2013 Master of Science in RegulatoryRhode Island College Providence, RI May 1998 Bachelor of Arts in Biology
Aug 2008 to May 2013 Graduate Teaching AssistantCleveland State University Cleveland, OH Aug 2008 to 2013 Research AssistantWal-Mart Incorporated North Olmsted, OH Jun 2007 to Aug 2008 Electronics Sales AssociateWal-Mart Incorporated Seekonk, MA Sep 2006 to Jun 2007 Electronics Sales AssociateTryzub Inc Cleveland, OH Feb 2003 to Jul 2006 Veterinary Technical AssistantGreenwich Valley Veterinary Clinic West Warwick, RI Jun 1998 to Dec 2002 Veterinary Technical Assistant
Education:
Cleveland State University Cleveland, OH May 2013 Masters of Science in Cell BiologyRhode Island College Providence, RI May 1998 Bachelor of Arts in Biology
Skills:
Extensive use of light microscopes PCR, agarose gel electrophoresis, Southern Hybridization. Extraction of genomic DNA (mitotic and meiotic) and plasmids from both E. coli and S. cerevisiae. Restriction digestion of DNA (plasmid and genomic) and designing of PCR primers (using software). Transformation of E. coli and S. cerevisiae with circular plasmids and linear DNA (PCR products). Prevention of self-ligation of restriction fragment with Antarctic phosphatase. Amplification of random S. Cerevisiae genomic library in competent DH5 alpha E. coli. Preparation of solid and liquid growth media and culturing of cells from both S. cerevisiae and E. coli using aseptic techniques. Culturing of both S. cerevisiae and E. Coli on various selection media (antibiotic and nutritional markers) to establish strain identification and selection for plasmid containing colonies. Induction of meiosis (sporulation) in S. Cerevisiae strains. Tetrad dissection (sporulated S. Cerevisiae cells) using light microscopy. Topo 2.1 cloning Passed HeLa cells Isolation of clotting factor V