Sasi Kumar Pillai

US Patent:

7307169, Dec 11, 2007

Filed:

Jan 5, 2004

Appl. No.:

10/751388

Inventors:

Darryl J. C. Pappin - Boxborough MA, US
Sasi Pillai - Littleton MA, US
James M. Coull - Westford MA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07D 241/04

US Classification:

544358, 544392, 544398, 544399

Abstract:

In some embodiments, this invention pertains to isotopically enriched N-substituted piperazines. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazines.

US Patent:

7355045, Apr 8, 2008

Filed:

Jan 5, 2004

Appl. No.:

10/751387

Inventors:

Subhakar Dey - Billerica MA, US
Darryl J. C. Pappin - Boxborough MA, US
Subhasish Purkayastha - Acton MA, US
Sasi Pillai - Littleton MA, US
James M. Coull - Westford MA, US

Assignee:

Applera Corporation - Framingham MA

International Classification:

C07D 241/04

US Classification:

544399

Abstract:

In some embodiments, this invention pertains to isotopically enriched N-substituted piperazine acetic acids. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazine acetic acids.

US Patent:

7364911, Apr 29, 2008

Filed:

May 14, 2002

Appl. No.:

10/477619

Inventors:

Rudolf H. Aebersold - Mercer Island WA, US
Huilin Zhou - San Diego CA, US
Beate Rist - 31180Giesen, DE
George J. Vella - Medway MA, US
Subhasish Purkayastha - Acton MA, US
Sasi Pillai - Littleton MA, US

International Classification:

G01N 24/00
G01N 21/76
G01N 33/68
G01N 33/92

US Classification:

436 86, 435 6, 436 71, 436172, 436173, 436501, 530402, 530405

Abstract:

The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.

US Patent:

7932388, Apr 26, 2011

Filed:

Dec 11, 2007

Appl. No.:

12/001734

Inventors:

Darryl J. C. Pappin - Boxborough MA, US
Sasi Pillai - Littleton MA, US
James M. Coull - Westford MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

C07D 241/04
C07D 295/067

US Classification:

544358, 544392, 544398, 544399

Abstract:

In some embodiments, this invention pertains to isotopically enriched N-substituted piperazines. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazines.

US Patent:

8236565, Aug 7, 2012

Filed:

May 29, 2007

Appl. No.:

11/754929

Inventors:

Subhasish Purkayastha - Acton MA, US
Subhakar Dey - N. Billerice MA, US
Sasi Pillai - Littleton MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

G01N 37/00

US Classification:

436 56, 560330, 544 59, 544171, 544399, 546248

Abstract:

In various aspects, the present teachings provide labeling reagents and sets of labeling reagents for the relative quantitation, absolute quantitation, or both, of hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols. In various aspects, the present teachings also provide methods for the analysis hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols my MS/MS methods.

US Patent:

8501498, Aug 6, 2013

Filed:

Nov 13, 2009

Appl. No.:

12/618452

Inventors:

Xiongwei Yan - Dublin CA, US
Pau-Miau Yuan - San Jose CA, US
Sylvia W. Yuen - San Mateo CA, US
Kuo-Liang Hsi - Fremont CA, US
Joe Y. Lam - Castro Valley CA, US
Krishna G. Upadhya - Union City CA, US
Subhakar Dey - North Billerica MA, US
Darryl J. C. Pappin - Boxborough MA, US
Sasi Pillai - Littleton MA, US
Helena Huang - Boxborough MA, US
Subhasish Purkayastha - Acton MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

G01N 33/532
G01N 33/534
G01N 33/533
G01N 24/00

US Classification:

436544, 436545, 436546, 436173

Abstract:

This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

US Patent:

8569304, Oct 29, 2013

Filed:

Mar 5, 2009

Appl. No.:

12/398920

Inventors:

Subhakar Dey - Billerica MA, US
Darryl J. C. Pappin - Boxborough MA, US
Subhasish Purkayastha - Acton MA, US
Sasi Pillai - Littleton MA, US
James M. Coull - Westford MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

A61K 31/497
C07D 241/04
C07D 295/00
C07D 403/00

US Classification:

51425212, 51425401, 544358, 544372

Abstract:

In some embodiments, this invention pertains to active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof. In some embodiments, this invention pertains to methods for the preparation of active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof.

US Patent:

8617898, Dec 31, 2013

Filed:

Jan 25, 2011

Appl. No.:

13/013664

Inventors:

Subhakar Dey - N. Billerica MA, US
Sasi K. Pillai - Littleton MA, US
Brian Williamson - Ashland MA, US
Subhasish Purkayastha - Acton MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

G01N 33/00
G01N 30/72

US Classification:

436131, 436 71, 436127, 436128, 436161, 436173, 436174, 436177, 436178, 436181

Abstract:

Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.