Darryl J. C. Pappin - Boxborough MA, US Sasi Pillai - Littleton MA, US James M. Coull - Westford MA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
C07D 241/04
US Classification:
544358, 544392, 544398, 544399
Abstract:
In some embodiments, this invention pertains to isotopically enriched N-substituted piperazines. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazines.
Isotopically Enriched N-Substituted Piperazine Acetic Acids And Methods For The Preparation Thereof
Subhakar Dey - Billerica MA, US Darryl J. C. Pappin - Boxborough MA, US Subhasish Purkayastha - Acton MA, US Sasi Pillai - Littleton MA, US James M. Coull - Westford MA, US
Assignee:
Applera Corporation - Framingham MA
International Classification:
C07D 241/04
US Classification:
544399
Abstract:
In some embodiments, this invention pertains to isotopically enriched N-substituted piperazine acetic acids. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazine acetic acids.
Methods For Isolating And Labeling Sample Molecules
Rudolf H. Aebersold - Mercer Island WA, US Huilin Zhou - San Diego CA, US Beate Rist - 31180Giesen, DE George J. Vella - Medway MA, US Subhasish Purkayastha - Acton MA, US Sasi Pillai - Littleton MA, US
The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.
Isotopically Enriched N-Substituted Piperazines And Methods For The Preparation Thereof
Darryl J. C. Pappin - Boxborough MA, US Sasi Pillai - Littleton MA, US James M. Coull - Westford MA, US
Assignee:
DH Technologies Development Pte. Ltd. - Singapore
International Classification:
C07D 241/04 C07D 295/067
US Classification:
544358, 544392, 544398, 544399
Abstract:
In some embodiments, this invention pertains to isotopically enriched N-substituted piperazines. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazines.
Tagging Reagents And Methods For Hydroxylated Compounds
Subhasish Purkayastha - Acton MA, US Subhakar Dey - N. Billerice MA, US Sasi Pillai - Littleton MA, US
Assignee:
DH Technologies Development Pte. Ltd. - Singapore
International Classification:
G01N 37/00
US Classification:
436 56, 560330, 544 59, 544171, 544399, 546248
Abstract:
In various aspects, the present teachings provide labeling reagents and sets of labeling reagents for the relative quantitation, absolute quantitation, or both, of hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols. In various aspects, the present teachings also provide methods for the analysis hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols my MS/MS methods.
Xiongwei Yan - Dublin CA, US Pau-Miau Yuan - San Jose CA, US Sylvia W. Yuen - San Mateo CA, US Kuo-Liang Hsi - Fremont CA, US Joe Y. Lam - Castro Valley CA, US Krishna G. Upadhya - Union City CA, US Subhakar Dey - North Billerica MA, US Darryl J. C. Pappin - Boxborough MA, US Sasi Pillai - Littleton MA, US Helena Huang - Boxborough MA, US Subhasish Purkayastha - Acton MA, US
Assignee:
DH Technologies Development Pte. Ltd. - Singapore
International Classification:
G01N 33/532 G01N 33/534 G01N 33/533 G01N 24/00
US Classification:
436544, 436545, 436546, 436173
Abstract:
This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.
Active Esters Of N-Substituted Piperazine Acetic Acids, Including Isotopically Enriched Versions Thereof
Subhakar Dey - Billerica MA, US Darryl J. C. Pappin - Boxborough MA, US Subhasish Purkayastha - Acton MA, US Sasi Pillai - Littleton MA, US James M. Coull - Westford MA, US
Assignee:
DH Technologies Development Pte. Ltd. - Singapore
International Classification:
A61K 31/497 C07D 241/04 C07D 295/00 C07D 403/00
US Classification:
51425212, 51425401, 544358, 544372
Abstract:
In some embodiments, this invention pertains to active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof. In some embodiments, this invention pertains to methods for the preparation of active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof.
Quantitative Analysis Of Vitamin D3, Vitamin D2, And Metabolites Thereof
Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.
Northeastern University 1995 - 1997
University of Geneva 1991 - 1992
The Maharaja Sayajirao University of Baroda 1986 - 1991
Doctorates, Doctor of Philosophy
University of Geneva
Northeastern University
Skills:
Mass Spectrometry Chemistry Proteomics R&D Hplc Small Molecules Biotechnology Laboratory Chromatography Peptide Synthesis Lc Ms Life Sciences Organic Chemistry Organic Synthesis Lifesciences Biochemistry Validation Commercialization High Performance Liquid Chromatography Research and Development Protein Purification Standard Operating Procedure Nuclear Magnetic Resonance Protein Chemistry Analytical Chemistry Ftir