Skills:
TECHNICAL SKILLS Behavioral Neuroscience: Assays to measure anxiety, fear acquisition and memory, stress reactivity, spatial and non-spatial learning and memory, sensorimotor gating, maternal and social behaviors and locomotor activity in rodents Molecular Neuroscience: PCR, reverse transcription PCR, Western blot, isolation of DNA, RNA and protein Neuroendocrine Techniques: Trunk and tail clip blood collection, acute and chronic stress paradigms, prenatal and postnatal stress, radioimmunoassay, estrous cycle tracking, collection of uteri, ovaries and testes Basic Neuroanatomical Methods: Cryosectioning, histological staining, light and fluorescence microscopy Animal Care and Use: Animal handling techniques to minimize hormonal stress response, small animal surgery, live decapitation and perfusion euthanasia techniques, intraperitoneal and subcutaneous injections Laboratory Management: Established and maintained a breeding colony of transgenic rats, trained laboratory technicians and student research assistants, managed laboratory supplies and supporting materials Data Analysis and Acquisition: SPSS versions 12.0 and 13.0, GraphPad Prism, AIS version 6.0, Any-MAZE behavioral tracking software, FreezeFrame software