Saadyah E Averick

US Patent:

20130190256, Jul 25, 2013

Filed:

Feb 25, 2011

Appl. No.:

13/581020

Inventors:

Krishnaswami Raja - Staten Island NY, US
Alejandra Alonso - Staten Island NY, US
Probal Banerjee - Staten Island NY, US
Sukanta Dolai - Staten Island NY, US
Christopher Corbo - Staten Island NY, US
Saadyah Averick - Staten Island NY, US
Amit Mogha - Staten Island NY, US
Shawon Debnath - Staten Island NY, US

Assignee:

Research Foundation of the City University of New York - New York NY

International Classification:

C07H 13/04
C07H 7/06

US Classification:

514 25, 536 174, 536 181

Abstract:

The invention relates to novel curcumin derivatives in which one or two of the phenolic groups have been modified.

US Patent:

20130261259, Oct 3, 2013

Filed:

Mar 7, 2013

Appl. No.:

13/788710

Inventors:

Krzysztof Matyjaszewski - Pittsburgh PA, US
Saadyah Averick - Pittsburgh PA, US

Assignee:

FRANKLIN AND MARSHALL COLLEGE - Lancaster PA

International Classification:

C07C 229/36
C07C 247/12
C08F 289/00
C07C 231/14
C07K 14/435
C07C 233/81
C07C 247/10

US Classification:

525 541, 562449, 560228, 552 10, 560142, 552 12, 530350, 435 681

Abstract:

This invention pertains to methods for producing homogeneous recombinant proteins that contain polymer initiators at defined sites. The unnatural amino acid, 4-(2′-bromoisobutyramido)phenylalanine of formula 1, was designed and synthesized as a molecule comprising a functional group further comprising an initiator for an atom-transfer radical polymerization (‘ATRP”) that additionally would provide a stable linkage between the protein and growing polymer. We evolved a (Mj) tyrosyl-tRNA synthetase/tRNApair to genetically encode this unnatural amino acid in response to an amber codon. To demonstrate the utility of this functional amino acid, we produced Green Fluorescent Protein with the unnatural amino acid initiator of formula 1 site-specifically incorporated on its surface (GFP-1). Purified GFP-1 was then used as an initiator under standard ATRP conditions with oligo(ethylene oxide)monomethyl ether methacrylate, efficiently producing a polymer-GFP bioconjugate wherein the polymer is connected at a specifically selected site on GFP.

US Patent:

20140004591, Jan 2, 2014

Filed:

Mar 15, 2013

Appl. No.:

13/837590

Inventors:

Krzysztof Matyjaszewski - Pittsburgh PA, US
Saadyah Averick - Pittsburgh PA, US

Assignee:

FRANKLIN AND MARSHALL COLLEGE - Lancaster PA

International Classification:

C08F 220/26
C12N 9/96

US Classification:

435188, 527202

Abstract:

The present invention is directed towards a protein-polymer composition having a protein with a site-specifically incorporated unnatural amino acid initiator and a covalently attached polymer.

US Patent:

20110262991, Oct 27, 2011

Filed:

May 18, 2009

Appl. No.:

12/992854

Inventors:

Krishnaswami Raja - Staten Island NY, US
Wei Shi - Staten Island NY, US
Sukanta Dolai - Staten Island NY, US
Saadyah Averick - Staten Island NY, US
William L'Amoreaux - Freehold NJ, US
Probal Banerjee - Staten Island NY, US

International Classification:

C12N 9/96
C12N 7/00
C08F 120/06

US Classification:

435188, 525 542, 525348, 525351, 525 541, 4352351

Abstract:

Water soluble polymers having formula I: Y-(L)-(C(O))—(R)—Rare claimed. The polymers may contain multiple water soluble, immunogenicity reducing moieties and multiple active moieties. The polymers may be linked to a protein, or a peptide having up to twelve amino acids.

US Patent:

20180142288, May 24, 2018

Filed:

Nov 2, 2017

Appl. No.:

15/802066

Inventors:

- Pittsburgh PA, US
Krzysztof Matyjaszewski - Pittsburgh PA, US
Bruce A. Armitage - Pittsburgh PA, US
Subha Ranjan Das - Pittsburgh PA, US
Saadyah Averick - Pittsburgh PA, US

International Classification:

C12Q 1/6834
C07H 21/04
C08L 57/00

Abstract:

A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

US Patent:

20170158836, Jun 8, 2017

Filed:

Nov 25, 2014

Appl. No.:

15/039219

Inventors:

- Pittsburgh PA, US
Saadyah Averick - Pittsburgh PA, US
Hongkun He - Pittsburgh PA, US

International Classification:

C08J 9/26
C02F 3/34
C12N 9/96
C08J 9/00
C08J 9/36

Abstract:

A three-dimensionally ordered macroporous hydrogel for immobilizing a selected bioresponsive molecule and method of making are disclosed. The three-dimensionally ordered macroporous hydrogel comprises a crosslinked polymer that has a system of interconnected pores. The interconnected pores have a uniform pore size in the range of 50 to 5000 nm, and a plurality of first pore functional groups. The plurality of first pore functional groups is selected to immobilize a selected bioresponsive molecule. Examples of bioresponsive molecules include an enzyme; a molecule for: a protein scaffold, solid phase synthesis, nucleic acid synthesis, polypeptide synthesis, analyte detection, adsorption of analytes and measuring analyte concentrations, organic synthesis, and degradation of biologically active agents in wastewater. A method includes forming a colloidal crystal template, polymerizing a hydrogel within the pores of the colloidal crystal template, and selectively removing the colloidal crystal template. The hydrogel can be polymerized using CRP, ATRP and FRP polymerization processes.

US Patent:

20170152336, Jun 1, 2017

Filed:

Apr 17, 2014

Appl. No.:

14/785334

Inventors:

- Pittsburgh PA, US
SAADYAH AVERICK - Pittsburgh PA, US
SOURAV K. DEY - Pittsburgh PA, US
Krzysztof Matyjaszewski - Pittsburgh PA, US

International Classification:

C08F 251/00

Abstract:

A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.

US Patent:

20170145048, May 25, 2017

Filed:

Dec 14, 2016

Appl. No.:

15/378537

Inventors:

- Pittsburgh PA, US
Saadyah E. Averick - Pittsburgh PA, US
Antonina Simakova - Pittsburgh PA, US

International Classification:

C07K 1/107
C07K 14/765
C07K 14/435
C08G 81/02
C08F 283/06

Abstract:

Methods for conducting controlled grafting-from radical polymerizations from biomolecules under conditions that are biologically compatible are described. The methods provide biomolecule-polymer conjugates with highly controlled structures and narrow polydispersities under aqueous reaction conditions and biological temperatures. Biomolecules, such as proteins and nucleotides can be conjugated to polymers with high levels of control.