Rolfe Sholeh Anderson

US Patent:

6403317, Jun 11, 2002

Filed:

Mar 24, 2000

Appl. No.:

09/535142

Inventors:

Rolfe C. Anderson - Saratoga CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 4352872, 4352887, 422 681, 422 8201, 422 8205, 422 8212

Abstract:

A method of detecting locations on a nucleic acid probe array at which hybridization occurs between targets in a fluid sample and nucleic acid probes disposed on a surface of the nucleic acid probe array, comprising: measuring the temperature at a plurality of locations on the surface of the nucleic acid probe array; applying an oscillating level of energy to the surface of the nucleic acid probe array, thereby causing the temperature at the surface of the nucleic acid probe array to oscillate; and detecting a decreased range of temperature oscillation at least one of the plurality of locations on the nucleic acid probe array, thereby indicating an increased heat capacity caused by latent heat of hybridization between at least one target in the fluid sample and at least one nucleic acid probe disposed on a surface of the nucleic acid probe array.

US Patent:

6830936, Dec 14, 2004

Filed:

Dec 31, 2000

Appl. No.:

09/751658

Inventors:

Rolfe C. Anderson - Saratoga CA
Robert J. Lipshutz - Palto Alto CA
Richard P. Rava - San Jose CA
Stephen P. A. Fodor - Palo Alto CA

Assignee:

Affymetrix Inc. - Santa Clara CA

International Classification:

G01N 3510

US Classification:

436180, 422100, 4352872, 4352865, 4352885

Abstract:

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.

US Patent:

6864050, Mar 8, 2005

Filed:

Aug 16, 1999

Appl. No.:

09/373984

Inventors:

Xing Su - Cupertino CA, US
Rolfe C. Anderson - Saratoga CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q001/68
C12P019/34
C07H021/04

US Classification:

435 6, 435 912, 435 9151, 536 243

Abstract:

The amplification of nucleic acids in a single phase can reliably provide products at relatively low cost and labor. A single-phase amplification can also increase the amount of nucleic acids while preserving the relative abundance of the individual nucleic acid species, or portions thereof. A single-phase amplified nucleic acid preparation may be analyzed in a gene expression monitoring system, preferably involving a nucleic acid probe array.

US Patent:

20020022261, Feb 21, 2002

Filed:

Dec 31, 2000

Appl. No.:

09/751657

Inventors:

Rolfe Anderson - Saratoga CA, US
Robert Lipshutz - Palo Alto CA, US
Richard Rava - Redwood City CA, US
Stephen Fodor - Palo Alto CA, US

International Classification:

C12M001/34

US Classification:

435/287200, 435/006000, 435/287900, 435/288600

Abstract:

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system.

US Patent:

20020058329, May 16, 2002

Filed:

Feb 16, 2001

Appl. No.:

09/788209

Inventors:

Sharat Singh - San Jose CA, US
Liching Cao - Vallejo CA, US
Herbert Hooper - Belmont CA, US
David Albagli - Millbrae CA, US
Rolfe Anderson - Saratoga CA, US
Shulin Zeng - Sunnyvale CA, US

International Classification:

C12M001/34

US Classification:

435/287200, 435/288500, 435/006000

Abstract:

A method and device for performing a plurality of small-volume reactions simultaneously are disclosed. The device includes an elongate or planar channel and a port for introducing such bulk-phase medium into the channel, a plurality of discrete small-volume reaction regions within the channel, and a reaction-specific reagent releasably carried on a wall portion of each reaction region. In carrying out the method of the invention, a bulk phase medium containing common reactants is added to the channel. Upon release of reaction-specific reagent from the wall portions of the reaction regions, a reagent-specific reaction can occur simultaneously in each region. The channel is dimensioned to substantially prevent convective fluid flow among the reaction regions during such reactions.

US Patent:

20020172975, Nov 21, 2002

Filed:

Jul 8, 2002

Appl. No.:

10/191877

Inventors:

Rolfe Anderson - Saratoga CA, US

Assignee:

AFFYMETRIX, INC. - Santa Clara CA

International Classification:

C12Q001/68
G01K003/00
C12M001/34

US Classification:

435/006000, 435/287200, 374/137000

Abstract:

A method of detecting locations on a nucleic acid probe array at which hybridization occurs between targets in a fluid sample and nucleic acid probes disposed on a surface of the nucleic acid probe array, comprising: measuring the temperature at a plurality of locations on the surface of the nucleic acid probe array; applying an oscillating level of energy to the surface of the nucleic acid probe array, thereby causing the temperature at the surface of the nucleic acid probe array to oscillate; and detecting a decreased range of temperature oscillation at at least one of the plurality of locations on the nucleic acid probe array, thereby indicating an increased heat capacity caused by latent heat of hybridization between at least one target in the fluid sample and at least one nucleic acid probe disposed on a surface of the nucleic acid probe array.

US Patent:

20030017467, Jan 23, 2003

Filed:

Aug 23, 2001

Appl. No.:

09/938439

Inventors:

Herbert Hooper - Belmont CA, US
Sharat Singh - San Jose CA, US
Travis Boone - San Mateo CA, US
Rolfe Anderson - Saratoga CA, US
David Albagli - Millbrae CA, US
Antonio Ricco - Los Gatos CA, US

Assignee:

Aclara BioSciences, Inc.

International Classification:

G01N033/00

US Classification:

435/006000, 436/180000, 422/058000, 422/100000

Abstract:

A microchannel apparatus and method for processing a sample are disclosed. The apparatus include a multisite reaction channel, one or more sample-preparation stations upstream of the reaction channel, for carrying out one or more selected sample-preparation steps effective to convert a sample to the bulk-phase medium, and one or more product-processing stations downstream of the reaction channel, for processing products generated in one or more of the reaction regions. Also included is structure for transferring solvent or solvent components between one of the sample-preparation stations and one or more selected reaction regions in the reaction channel, and between one or more selected reaction regions in the reaction channel and one of said product-processing stations, under the control of a control unit.

US Patent:

20030027352, Feb 6, 2003

Filed:

Aug 23, 2001

Appl. No.:

09/938433

Inventors:

Herbert Hooper - Belmont CA, US
Sharat Singh - San Jose CA, US
Travis Boone - San Mateo CA, US
Rolfe Anderson - Saratoga CA, US
David Albagli - Millbrae CA, US
Antonio Ricco - Los Gatos CA, US

Assignee:

Aclara BioSciences, Inc.

International Classification:

G01N031/22

US Classification:

436/169000, 422/058000, 422/068100

Abstract:

A method and apparatus for performing a plurality of small-volume reactions simultaneously with components in a bulk-phase medium are disclosed. The apparatus includes a device having an elongate or planar channel providing a plurality of discrete small-volume reaction regions within the channel, each communicating with supply and hold reservoirs from which material can be introduced into the regions, and to which material can be transferred from the reaction regions. A reaction-specific reagent is carried on a wall portion of each reaction region, or in a reservoir associated with a reaction region, for reacting in solution with one or more components in the bulk-phase medium, to effect a selected solution-phase reaction in each region or in a region-specific reservoir associated therewith. The channel or the fluid communication between a reaction region and an associated region-specific region is dimensioned to substantially prevent convective fluid flow among the reaction regions during such reactions, thus confining the reaction in each region. The apparatus also includes structure for moving solvent or solvent components from or to a selected region-specific reservoir, to or from the associated reaction region, and a control unit for controlling such reservoir-to channel and channel-to-reservoir movement.