Sheila M. Colby - Sunnyvale CA John E. Crock - Moscow ID Peggy G. Lemaux - Moraga CA Rodney B. Croteau - Pullman WA
Assignee:
The Regents of the University of California - Berkley CA Washington State Research Foundation - Pullman WA
International Classification:
C12N 900
US Classification:
435183, 435 691, 435419, 536 232
Abstract:
Germacrene C synthase genes from have been cloned and sequenced. Transgenic expression of germacrene C synthase in plants can result in beneficial and useful characteristics such as increased host resistance to pathogens and herbivores and altered flavor and odor profiles.
1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerases, And Methods Of Use
Rodney B. Croteau - Pullman WA Bernd M. Lange - Pullman WA
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12N 990
US Classification:
435233
Abstract:
The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy- -xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy- -xylulose-5-phosphate reductoisomerase protein from peppermint ( ). Additionally, the present invention relates to isolated plant 1-deoxy- -xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy- -xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.
Monoterpene Synthases From Grand Fir (Abies Grandis)
cDNAs encoding gymnosperm monoterpene synthases have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a monoterpene synthase of the invention. Thus, systems and methods are provided for the recombinant expression of recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts.
Sesquiterpene Synthases From Grand Fir (Abies Grandis), And Methods Of Use
Rodney Bruce Croteau - Pullman WA Jörg Bohlmann - Jena, DE John E. Crock - Moscow ID Christopher L. Steele - Admore OK
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12N 988
US Classification:
435232, 536 232
Abstract:
cDNAs encoding, E--bisabolene synthase, -selinene synthase and -humulene synthase from Grand Fir ( ) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E--bisabolene synthase (SEQ ID No:13), -selinene synthase (SEQ ID No:20) and -humulene synthase (SEQ ID No:24), respectively, from Grand Fir ( ). In other aspects, replicable recombinant cloning vehicles are provided which code for E--bisabolene synthase, -selinene synthase and -humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E--bisabolene synthase, -selinene synthase or -humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E--bisabolene synthase, -selinene synthase or ,-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene syntheses that may be used to facilitate their production, isolation and purification in significant amounts.
The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in cells.
Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxolâ), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
Nucleic Acid Molecules Encoding 10-Deacetylbaccatin Iii-O-Acetyl Transferase And Related Products
Transacylase enzymes of and the use of such enzymes to produce Taxolâ, related taxoids, as well as intermediates in the Taxolâ biosynthetic pathway are disclosed. Examples of specific enzymes described herein include taxadienol 5-O-acetyl transacylase (TAX1) and 10-deacetylbaccatin III-10-O-acetyl transferase (TAX6). Also disclosed are nucleic acid sequences encoding the transacylase enzymes.
Isolation And Bacterial Expression Of A Sesquiterpene Synthase Cdna Clone From Peppermint (Mentha X Piperita, L.) That Produces The Aphid Alarm Pheromone (E)-Β-Farnesene
A cDNA encoding (E)-β-farnesene synthase from peppermint () has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-β-farnesene synthase (SEQ ID NO:2), from peppermint (). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-β-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-β-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-β-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-β-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-β-farnesene synthase may be used to obtain expression or enhanced expression of (E)-β-famesene synthase in plants in order to enhance the production of (E)-β-farnesene, or may be otherwise employed for the regulation or expression of (E)-β-farnesene synthase, or the production of its product.