Craig Cummings - Pacifica CA, US Paolo Vatta - San Mateo CA, US Robert Tebbs - Livermore CA, US Priya Balachandran - Foster City CA, US Patrick Zoder - Millbrae CA, US Lily Wong - San Mateo CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68 C07H 21/04
US Classification:
435 611, 536 2432, 536 2433, 435 612
Abstract:
Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of from each other, for example may be detected independently of other spp. Some embodiments also describe a duplexed assay that can detect (formerly incertae-sedis), , and . Kits for detection of are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN assay. In some embodiments, 0.2-2 cfu of spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.
Inducible Nucleic Acid Targets For Detection Of Pathogens, Methods And Compositions Thereof
Priya Balachandran - Foster City CA, US Craig Cummings - Pacifica CA, US Hemant Pawar - Ann Arbor MI, US Robert Tebbs - Livermore CA, US Manohar Furtado - San Ramon CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68
US Classification:
435 611, 435 615, 435 612
Abstract:
The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, spp, spp. and spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.
Detection Of Salmonella Enterica Subspecies Enterica Serovar Enteritidis In Food And Environmental Samples, Methods And Compositions Therefor
Robert TEBBS - Livermore CA, US Craig Cummings - Pacifica CA, US Arlene Nunez - San Jose CA, US Priya Balachandran - Foster City CA, US Payman Fatemi - Pleasanton CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68
US Classification:
435 611, 435 612
Abstract:
Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of , subspecies , serovar () in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect without cross-reacting with C or other closely related species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.
Compositions And Methods For Detection Of Multiple Microorganisms
Robert TEBBS - Austin TX, US Craig Cummings - Pacifica CA, US Pius Brzoska - Woodside CA, US Sharon Matheny - Keller TX, US Catherine O'Connell - Austin TX, US Manohar Furtado - San Ramon CA, US Rixun Fang - Menlo Park CA, US Michael Schumaker - San Mateo CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68
US Classification:
435 612, 536 2433
Abstract:
The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.
Pius Brzoska - Woodside CA, US Somaya Bit - San Ramon CA, US Jen-Kuei Liu - Palo Alto CA, US Robert Tebbs - Livermore CA, US Manohar Furtado - San Ramon CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68 C07H 21/04
US Classification:
435 6, 536 2433
Abstract:
Disclosed is a multi-primer amplification assay, method and kits for detecting species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates in the sample.
Compositions, Kits And Methods For Detection Of Viral Sequences
- Carlsbad CA, US Ioanna PAGANI - Oakland CA, US Pius BRZOSKA - Woodside CA, US Jisheng LI - Pleasanton CA, US Chunling WANG - Redwood City CA, US Kathleen HAYASHIBARA - Cupertino CA, US Michael TANNER - South San Francisco CA, US Hong JI - Danville CA, US Kamini VARMA - Saratoga CA, US Fangqi HU - Palo Alto CA, US Robert TEBBS - Austin TX, US
International Classification:
C12Q 1/70
Abstract:
Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.
Compositions, Kits And Methods For Detection Of Viral Sequences
- Carlsbad CA, US Ioanna PAGANI - Oakland CA, US Pius BRZOSKA - Woodside CA, US Jisheng LI - Pleasanton CA, US Chunling WANG - Redwood City CA, US Kathleen HAYASHIBARA - Cupertino CA, US Michael TANNER - South San Francisco CA, US Hong JI - Danville CA, US Kamini VARMA - Saratoga CA, US Fangqi HU - Palo Alto CA, US Robert TEBBS - Austin TX, US
International Classification:
C12Q 1/70
Abstract:
Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.
Compositions, Kits And Methods For Detection Of Viral Sequences
- Carlsbad CA, US Ioanna PAGANI - Oakland CA, US Pius BRZOSKA - Woodside CA, US Jisheng LI - Pleasanton CA, US Chunling WANG - Redwood City CA, US Kathleen HAYASHIBARA - Cupertino CA, US Michael TANNER - South San Francisco CA, US Hong JI - Danville CA, US Kamini VARMA - Saratoga CA, US Fangqi HU - Palo Alto CA, US Robert TEBBS - Austin TX, US
International Classification:
C12Q 1/70
Abstract:
Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-CoV-2 and/or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.
Thermo Fisher Scientific Oct 2004 - 2010
Senior Staff Scientist
Life Technologies Jul 2006 - Apr 2009
Scientist
Life Technologies Oct 2004 - Jul 2006
Scientist Iii
Lawrence Livermore National Laboratory 2000 - 2004
Biomedical Scientist
Ucsf 1997 - 2000
Assistant Research Biologist
Education:
Wayne State University 1985 - 1991
Doctorates, Doctor of Philosophy, Biochemistry
Southern Utah University 1979 - 1985
Bachelors, Bachelor of Science, Mathematics, Chemistry
Panguitch High School
Skills:
Molecular Biology Assay Development Qpcr Biotechnology Validation R&D Genomics Dna Sequencing Dna Biochemistry Real Time Pcr Life Sciences Food Food Pathogen Testing Pcr Sequencing Dna Repair Lifesciences Immunoassays Genetics Protein Chemistry Bioinformatics Microbiology Purification Real Time Polymerase Chain Reaction Polymerase Chain Reaction Commercialization