Robert J Lipert

US Patent:

7179383, Feb 20, 2007

Filed:

Mar 4, 2004

Appl. No.:

10/793058

Inventors:

Marc D Porter - Ames IA, US
Jing Ni - San Jose CA, US
G Brent Dawson - San Jose CA, US
Ruth Shinar - Ames IA, US
Robert J Lipert - Ames IA, US
Michael C Granger - Gilbert IA, US
Mark Tondra - Minneapolis MN, US

Assignee:

Iowa State University Research Foundation - Ames IA

International Classification:

B03C 1/00
G01N 33/553

US Classification:

210695, 210746, 210222, 210243, 436149, 436150, 436526, 436806

Abstract:

Method and apparatus for manipulating and monitoring analyte flowing in fluid streams. A giant magnetoresistive sensor has an array of sensing elements that produce electrical output signals which vary in dependence on changes in the magnetic field proximate the sensing elements. The analyte is included in a stream, such that the stream has a magnetic property which is dependent on the concentration and distribution on the analyte therein. The stream is flowed past the giant magnetoresistive sensor and in sufficiently close proximity to cause the magnetic properties of the stream to produce electrical output signals. The electrical output signals are monitored as an indicator of analyte concentration or distribution in the stream flowing past the GMR sensor. Changes in the magnetic field produced by the background stream are introduced by analyte molecules, whose presence in the flow past the GMR will effect the output reading.

US Patent:

7473529, Jan 6, 2009

Filed:

Mar 31, 2005

Appl. No.:

11/095098

Inventors:

Marc D. Porter - Ames IA, US
Robert J. Lipert - Ames IA, US
Robert T. Doyle - Ames IA, US
Desiree S. Grubisha - Corona CA, US
Salma Rahman - Ames IA, US

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

C12Q 1/68

US Classification:

435 6, 435 29

Abstract:

A system and method for detecting a detectably labeled cell or component thereof in a sample comprising one or more cells or components thereof, at least one cell or component thereof of which is detectably labeled with at least two detectable labels. In one embodiment, the method comprises: (i) introducing the sample into one or more flow cells of a flow cytometer, (ii) irradiating the sample with one or more light sources that are absorbed by the at least two detectable labels, the absorption of which is to be detected, and (iii) detecting simultaneously the absorption of light by the at least two detectable labels on the detectably labeled cell or component thereof with an array of photomultiplier tubes, which are operably linked to two or more filters that selectively transmit detectable emissions from the at least two detectable labels.

US Patent:

7604953, Oct 20, 2009

Filed:

May 30, 2008

Appl. No.:

12/130528

Inventors:

Marc D. Porter - Salt Lake City UT, US
Betsy Jean Yakes - Alexandria VA, US
Robert J. Lipert - Ames IA, US
John P. Bannantine - Ames IA, US

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA
The United States of America as represented by the Department of Agriculture/Cooperative State Research Education and Extension Service (USDA/CSREES) - Washington DC

International Classification:

G01N 33/53
C12Q 1/00
A61K 39/04

US Classification:

435 71, 435 4, 424 91, 424 92, 4241301, 4241631, 4241641, 4241781, 4242341, 4242481

Abstract:

A process of detection of the causative agent of Johne's disease (subsp. ) (MAP) by detecting shedding of surface protein of MAP. A preferred way is use of surface enhanced Raman Spectroscopy. The system of detecting MAP shedding of protein provides early detection and diagnosis, and therefore allows early treatment for Johne's disease in ruminant animals.

US Patent:

7824926, Nov 2, 2010

Filed:

Sep 24, 2001

Appl. No.:

09/961628

Inventors:

Marc D. Porter - Ames IA, US
Jing Ni - San Jose CA, US
Robert J. Lipert - Ames IA, US
G. Brent Dawson - San Jose CA, US

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

G01N 33/553

US Classification:

436525, 436173, 436164

Abstract:

The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i. e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e. g. , immunoassays, imaging, and drug screening).

US Patent:

7829348, Nov 9, 2010

Filed:

Aug 31, 2004

Appl. No.:

10/931142

Inventors:

Marc D. Porter - Ames IA, US
Jing Ni - San Jose CA, US
Robert J. Lipert - Ames IA, US
G. Brent Dawson - San Jose CA, US

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

G01N 33/553

US Classification:

436525, 436164, 436514

Abstract:

The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i. e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e. g. , immunoassays, imaging, and drug screening).

US Patent:

20080199880, Aug 21, 2008

Filed:

Feb 21, 2007

Appl. No.:

11/677464

Inventors:

MARC D. PORTER - AMES IA, US
JEREMY D. DRISKELL - AMES IA, US
ROBERT J. LIPERT - AMES IA, US

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

An improvement in heterogeneous immunoassays to significantly reduce assay time, from as much as 50% up to 90% of what used to be typical assay times. The improvement involves rotating the captured substrate during incubation times for antigen capture and during incubation times for sample labeling.

US Patent:

20090017562, Jan 15, 2009

Filed:

Mar 7, 2008

Appl. No.:

12/044551

Inventors:

Marc D. Porter - Salt Lake City UT, US
Hye-Young Park - Yongin Si Suji-gu, KR
Robert J. Lipert - Ames IA, US

Assignee:

IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. - Ames IA

International Classification:

G01N 33/544
G01N 33/543

US Classification:

436528, 436518

Abstract:

Raman Active Reagents (ERLs) are developed which use a nanoparticle substrate substantially covered with a mixed monolayer derived from a Raman active reporter molecule and an analyte binding molecule that both bind to the surface of the nanoparticle and thereby avoid the necessity for separate synthesis of a bifunctional linker molecule in making the ERL.

US Patent:

20110070661, Mar 24, 2011

Filed:

Sep 30, 2010

Appl. No.:

12/894693

Inventors:

MARC D. PORTER - Salt Lake City UT, US
JING NI - Sunnyvale CA, US
ROBERT J. LIPERT - AMES IA, US
G. BRENT DAWSON - Greensboro NC, US

Assignee:

IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. - AMES IA

International Classification:

G01N 33/53
G01N 21/00

US Classification:

436501, 436164

Abstract:

The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).