A cricothyroidotomy instrument having an elongated needle and a holder for the needle. The needle has a sharp point on one end, a flange on the other end and a longitudinal passage extending from the end of the needle with the sharp point to the end of the needle with the flange. The needle holder has two separable sections having faces which abut when the needle holder is closed and spaced annular grooves are formed in the abutting faces. A plurality of annular spaced beads is formed on the exterior surface of the needle which engage the annular grooves in the needle holder. A latch maintains the separable sections in the closed relationship to clamp the needle in the needle holder.