Richard L Klemke

US Patent:

20100242123, Sep 23, 2010

Filed:

Sep 28, 2007

Appl. No.:

12/445283

Inventors:

Yury Miller - San Diego CA, US
Konstantin Stoletov - San Diego CA, US
Richard Klemke - San Diego CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

G01N 33/00
A01K 67/00
C12N 5/00

US Classification:

800 3, 800 20, 435325

Abstract:

The invention provides genetically altered fish of the family Cyprinidae, or genus , including zebrafish () and host cells from these animals, where the fish have been genetically altered to lack or have a modified gene related to lipid metabolism, for example, an ApoE, ApoAI and/or LDL-R gene. In another aspect, the invention is directed to drug design or discovery using the animal or cell models of the invention and/or wild type zebrafish, and by administering an altered diet and/or environment to the animal of invention. The invention also provides methods for screening for a compound capable of ameliorating or preventing or reversing: atherosclerosis; hyperlipidemia; lipoprotein oxidation; the accumulation of lipid in a blood vessel wall; vascular inflammation associated with lipid accumulation in a blood vessel wall; acute atherosclerosis-associated events; heart attack; stroke.

US Patent:

20030224398, Dec 4, 2003

Filed:

Feb 12, 2003

Appl. No.:

10/365961

Inventors:

Richard Klemke - San Diego CA, US

International Classification:

C12Q001/00
C12Q001/68
G01N033/53
G01N033/567
A61K031/739
C12N005/06

US Classification:

435/006000, 435/004000, 435/007200, 435/317100, 435/325000, 514/054000

Abstract:

The invention relates generally to methods of modulating cell migration. Included in the methods of the invention are methods of identifying the state of a pseudopodium in cell migration and methods of inducing extension or retraction of a pseudopodia from a cell. The invention also relates to methods of screening for and identifying an agent effective in inducing extension or retraction of a pseudopodium and therefore affecting cell migration. Agents that can modulate cell migration are useful in treatment of conditions in which cell migration plays a role. Such conditions can include wound healing, angiogenesis, and metastasis of a disease from one location to another. Additionally, the invention provides methods of biochemically separating the pseudopodium of a cell from the remainder of the cell body and methods of determining the proteins present in the pseudopodium and cell body. The invention also includes a pseudopodium isolated by the methods of the invention.