Richard C Egen

Author:

Richard A. Egen

ISBN #:

0309018978

US Patent:

7399615, Jul 15, 2008

Filed:

Aug 16, 2005

Appl. No.:

11/204562

Inventors:

Richard C. Egen - Cheyney PA, US
Lori Ann Fortin - Bethlehem PA, US
Willie Wei Qiang Sun - Easton PA, US

International Classification:

C12P 19/04
C12N 1/20

US Classification:

435101, 435 711, 4352523, 536123

Abstract:

Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of serotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.

US Patent:

20040229319, Nov 18, 2004

Filed:

May 16, 2003

Appl. No.:

10/439878

Inventors:

Richard Egen - Cheyney PA, US
Lori Fortin - Bethlehem PA, US
Willie Wei Sun - Easton PA, US

International Classification:

C12P019/26
C12P019/04
C12N001/20

US Classification:

435/084000, 435/101000, 435/252100

Abstract:

Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of serotypes A, C, Y and W-135 and is run reproducibly at the 20 L scale and can be scaled to 400 L or more.

US Patent:

20080274515, Nov 6, 2008

Filed:

Jul 8, 2008

Appl. No.:

12/217683

Inventors:

Richard C. Egen - Cheyney PA, US
Lori Ann Fortin - Bethlehem PA, US
Willie Wei Qiang Sun - Easton PA, US

International Classification:

C12P 19/04
C12N 1/20

US Classification:

435101, 4352536, 4352521

Abstract:

Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of serotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.