Randolph V Lewis

Author:

Randolph Lewis

ISBN #:

0292776713

Author:

Randolph Lewis

ISBN #:

0299169103

Author:

Randolph Lewis

ISBN #:

0299169146

Author:

Randolph Lewis

ISBN #:

0803280459

US Patent:

7288391, Oct 30, 2007

Filed:

Jun 6, 2002

Appl. No.:

10/479638

Inventors:

Don A. Roth - Laramie WY, US
Randolph V. Lewis - Laramie WY, US

Assignee:

University of Wyoming - Laramie WY

International Classification:

C12P 21/06
A01H 9/00
C07K 14/00

US Classification:

435 691, 4353201, 800295, 530350

Abstract:

The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.

US Patent:

7521228, Apr 21, 2009

Filed:

Mar 28, 2002

Appl. No.:

10/488056

Inventors:

Randolph V. Lewis - Laramie WY, US
Cheryl Y. Hayashi - Riverside CA, US
John E. Gatesy - Riverside CA, US
Dagmara Motriuk - Laramie WY, US

Assignee:

The University of Wyoming - Laramie WY

International Classification:

C12N 15/00
C07K 14/00
C07H 21/02

US Classification:

4353201, 4352523, 435352, 530350, 530300, 536 231

Abstract:

Spider silk protein encoding nucleic acids, polypeptides and antibodies immunologically specific therefore are disclosed. Methods of use thereof are also provided.

US Patent:

7723109, May 25, 2010

Filed:

Oct 29, 2007

Appl. No.:

11/926871

Inventors:

Randolph V. Lewis - Laramie WY, US

Assignee:

University of Wyoming - Laramie WY

International Classification:

C12N 5/00
C12P 21/06
C07H 17/00
C07K 14/00

US Classification:

435410, 435325, 435243, 4353201, 530300, 530350, 536 231

Abstract:

The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.

US Patent:

8114631, Feb 14, 2012

Filed:

Sep 17, 2009

Appl. No.:

12/561779

Inventors:

Omer Choresh - Tel Aviv, IL
Randolph V. Lewis - Laramie WY, US

Assignee:

The University of Wyoming - Laramie WY

International Classification:

A61F 2/82
C07H 21/04
C07K 14/435

US Classification:

435 691, 424423, 623 142, 536 235

Abstract:

Nucleic acids encoding spider glue proteins and methods of use thereof are disclosed.

US Patent:

56959710, Dec 9, 1997

Filed:

Apr 7, 1995

Appl. No.:

8/418194

Inventors:

Yoichi Kadokami - Laramie WY
Randolph V. Lewis - Laramie WY

Assignee:

Amresco - Solon OH

International Classification:

C12N 1500
C12N 120
C07H 2104

US Classification:

4351721

Abstract:

A hybrid vector produced from a linearized cosmid and arms from. lambda. phage vector DNA has been developed. The hybrid vector can be used to generate fragments which are useful as vectors in a helper phage-mediated transformation system, permitting large fragments of foreign DNA to be introduced into a host on an industrial scale.

US Patent:

59898941, Nov 23, 1999

Filed:

Oct 4, 1994

Appl. No.:

8/317844

Inventors:

Randolph V. Lewis - Laramie WY
Ming Xu - Calgary, CA
Michael B. Hinman - Laramie WY

Assignee:

University of Wyoming - Laramie WY

International Classification:

C12N 121
C12N 1563
C07H 2104

US Classification:

4352523

Abstract:

The present invention is directed to isolated cDNA which codes for spider silk protein or a fragment or variant thereof, a replicable vector containing cDNA which codes for spider silk protein and which is capable of expressing spider silk protein, a transformed cell or microorganism containing cDNA which codes for spider silk protein or a fragment thereof which is capable of expressing spider silk protein and products, such as fibers, which may be manufactured utilizing the recombinant protein of the present invention.

US Patent:

59940999, Nov 30, 1999

Filed:

Jan 22, 1998

Appl. No.:

9/010928

Inventors:

Randolph V. Lewis - Laramie WY
Cheryl Y. Hayashi - Laramie WY

Assignee:

The University of Wyoming - Laramie WY

International Classification:

C12P 2106
C12N 1500
C07H 1700
C07K 1400

US Classification:

435 691

Abstract:

Cloned DNA encoding spider flagelliform silk proteins described. The translated amino acid sequence of the cloned cDNA shows that the flagelliform silk protein is composed largely of repeated motifs. The dominant motif of this protein is Gly-Pro-Gly-Gly-X, which can appear up to 63 times in tandem arrays. This motif likely forms Pro. sup. 2 -Gly. sup. 3 type II. beta. -turns. The resulting series of concatenated. beta. -turns are thought to form a helix (. beta. -spiral). The present inventors propose that this spring-like. beta. -spiral is the basis of the elasticity of silk.

US Patent:

57337713, Mar 31, 1998

Filed:

Mar 14, 1994

Appl. No.:

8/209747

Inventors:

Randolph V. Lewis - Laramie WY
Mark Colgin - Laramie WY

Assignee:

University of Wyoming - Laramie WY

International Classification:

C12N 121
C07H 2104
C07K 14435

US Classification:

4352523

Abstract:

cDNA clones encoding minor ampullate spidroin proteins (MiSP) are described. The translated amino acid sequence of the cloned cDNA shows that the MiSPs have a structure which exhibits an amino proximal nonrepetitive region, a repetitive portion and a carboxy-proximal nonrepetitive portion. The repetitive portion of the sequence is describable by a generic repeat formula. Comparison of the amino acid sequences derived from the translation with the sequences of short peptides obtained from solubilized minor ampullate spider silk suggests that the nonrepetitive portions of the protein are cleaved from the protein during secretion from the cells synthesizing the spidroins. This comparison also suggests that the minor ampullate spider silk is composed of at least three polypeptides.