Randall K Saiki from 1737 Alameda Ave, Alameda, CA 94501, age 70, Phone: 510 864-0202

Us Patents

Kits For Amplifying And Detecting Nucleic Acid Sequences
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US Patent:

6514736, Feb 4, 2003
Filed:

Nov 1, 2000
Appl. No.:

09/704357
Inventors:

Henry A. Erlich - Oakland CA
Glenn Horn - Emeryville CA
Randall K. Saiki - Richmond CA
Kary B. Mullis - La Jolla CA
David H. Gelfand - Oakland CA
Assignee:

Roche Molecular Systems, Inc - Alameda CA
International Classification:

C12N 912
US Classification:

435194, 435 6, 435 912, 536 231, 536 243
Abstract:

The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

Process For Discrimination Of Nucleic Acid Sequence Variants
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US Patent:

7141377, Nov 28, 2006
Filed:

Jan 27, 2005
Appl. No.:

11/043099
Inventors:

David H. Gelfand - Oakland CA, US
Pamela M. Holland - Seattle WA, US
Randall K. Saiki - Richmond CA, US
Robert M. Watson - Berkeley CA, US
Assignee:

Roche Molecular Systems, Inc. - Alameda CA
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435 6, 435 911, 435 912, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

Homogeneous Assay System
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US Patent:

7445900, Nov 4, 2008
Filed:

Aug 11, 2005
Appl. No.:

11/203461
Inventors:

David H. Gelfand - Oakland CA, US
Pamela M. Holland - Seattle WA, US
Randall K. Saiki - Richmond CA, US
Robert M. Watson - Berkeley CA, US
Assignee:

Roche Molecular Systems, Inc. - Pleasanton CA
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435 6, 435 912
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.

Homogeneous Assay System
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US Patent:

20060078914, Apr 13, 2006
Filed:

Aug 11, 2005
Appl. No.:

11/203040
Inventors:

David Gelfand - Oakland CA, US
Pamela Holland - Seattle CA, US
Randall Saiki - Richmond CA, US
Robert Watson - Berkeley CA, US
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435006000, 435091200
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.

Homogeneous Assay System
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US Patent:

20060084092, Apr 20, 2006
Filed:

Aug 11, 2005
Appl. No.:

11/203048
Inventors:

David Gelfand - Oakland CA, US
Pamela Holland - Seattle WA, US
Randall Saiki - Richmond CA, US
Robert Watson - Berkeley CA, US
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435006000, 435091200
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

Homogeneous Assay System
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US Patent:

20060257885, Nov 16, 2006
Filed:

Aug 11, 2005
Appl. No.:

11/203622
Inventors:

David Gelfand - Oakland CA, US
Pamela Holland - Seattle WA, US
Randall Saiki - Richmond CA, US
Robert Watson - Berkeley CA, US
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435006000, 435091200
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.

Kits For Amplifying And Detecting Nucleic Acid Sequences
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US Patent:

6197563, Mar 6, 2001
Filed:

Nov 18, 1994
Appl. No.:

8/345367
Inventors:

Henry A. Erlich - Oakland CA
Glenn Horn - Emeryville CA
Randall K. Saiki - Richmond CA
Kary B. Mullis - La Jolla CA
David H. Gelfand - Oakland CA
Assignee:

Roche Molecular Systems, Inc. - Pleasanton CA
International Classification:

C12N 912
US Classification:

435194
Abstract:

The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

Nucleic Acid Detection By The 5'-3'Exonuclease Activity Of Polymerases Acting On Adjacently Hybridized Oligonucleotides
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US Patent:

54879728, Jan 30, 1996
Filed:

Jan 5, 1993
Appl. No.:

7/961884
Inventors:

David H. Gelfand - Oakland CA
Pamela M. Holland - Seattle WA
Randall K. Saiki - Richmond CA
Robert M. Watson - Berkeley CA
Assignee:

Hoffmann-La Roche Inc. - Nutley NJ
International Classification:

C12Q 168
C12P 1934
US Classification:

435 6
Abstract:

A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

Wikipedia

Oligomer restricti

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OR was conceived by Randall Saiki and Henry Erlich in the early 1980s, working at Cetus Corporation in Emeryville, California. It was patented in 1984 and ...