Mark Powers - New Market MD, US Rafal Witek - Burlington NC, US Kirsten Amaral - Cary NC, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12N 5/071
US Classification:
435350, 435325, 435351, 435352, 435370, 4353052
Abstract:
Methods for preparing a variety of cell (e.g., hepatocyte) preparations and preparations so prepared are described. Uses of such preparations are described. In one embodiment, centrifugal elutriation is used to separate hepatocytes with preferred characteristics. Such hepatocyte preparations may be used for cryopreservation, multiple cryopreservations, in vitro assays, plating and other methods for which preparations of hepatocytes are useful.
Differentiated Cellular Compositions And Methods For Their Preparation And Use
- Carlsbad CA, US Stephen FERGUSON - Apex NC, US Rafal WITEK - Burlington NC, US
International Classification:
C12N 5/071
Abstract:
The present invention relates to methods for culturing progenitor cells to differentiate primarily into a single population of cells with the same phenotype, and to compositions thereof. In particular, it relates to methods for culturing liver progenitor cells to differentiate primarily into a single population of hepatocyte-like cells, and to compositions thereof.
Differentiated Cellular Compositions And Methods For Their Preparation And Use
- Carlsbad CA, US Stephen FERGUSON - Durham NC, US Rafal WITEK - Burlington NC, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12N 5/071
US Classification:
435377
Abstract:
The present invention relates to methods for culturing progenitor cells to differentiate primarily into a single population of cells with the same phenotype, and to compositions thereof. In particular, it relates to methods for culturing liver progenitor cells to differentiate primarily into a single population of hepatocyte-like cells, and to compositions thereof.
Ambys Medicines
Director of Cell Therapy Platform
Life Technologies Aug 2009 - Mar 2013
Hepatic Cell Biologist, Staff Scientist
Thermo Fisher Scientific Aug 2009 - Mar 2013
Senior Staff Scientist and Senior Manager and Associate Director of R and D, Adme and T
Duke University Health System Jul 2007 - Aug 2009
Senior Postdoctoral Research Associate and Nrsa Fellow Gastroenterology
University of Florida May 2005 - Jul 2007
Postdoctoral Research Associate
Education:
Duke University - the Fuqua School of Business 2012 - 2013
Master of Business Administration, Masters, Business
University of Florida 2001 - 2005
Doctorates, Doctor of Philosophy, Biology, Philosophy
The University of Georgia 1999 - 2000
Bachelors, Bachelor of Science, Biology, Microbiology
Skills:
Cell Culture Cell Cell Biology In Vitro Molecular Biology Assay Development Stem Cells Confocal Microscopy Life Sciences Biotechnology Laboratory In Vivo Molecular Cloning Qpcr Signal Transduction Lifesciences Flow Cytometry Drug Discovery Western Blotting Hepatocyte Isolation Dna Fluorescence Liver Npc Isolation Inflammation Genomics Laboratory Skills Rodent Survival Surgeries In Vitro Culture Rodent In Vivo Research Liver Hepatitis Acute Liver Failure Gene Therapy High Throughput Screening 3D Histotypic and Organotypic Liver Cultures Biochemistry Polymerase Chain Reaction