Peter M Gresshoff

Author:

Peter M. Gresshoff

ISBN #:

0471160679

Author:

Peter M. Gresshoff

ISBN #:

0849361885

Author:

Peter M. Gresshoff

ISBN #:

0849382610

Author:

Peter M. Gresshoff

ISBN #:

0849382637

Author:

Peter M. Gresshoff

ISBN #:

0849382645

Author:

Peter M. Gresshoff

ISBN #:

0849382653

US Patent:

54928109, Feb 20, 1996

Filed:

Feb 8, 1994

Appl. No.:

8/193514

Inventors:

Gustavo Caetano-Anolles - Knoxville TN
Brant J. Bassam - Knoxville TN
Peter M. Gresshoff - Knoxville TN

Assignee:

University of Tennessee Research Corporation - Knoxville TN

International Classification:

C12Q 168
G01N 2726

US Classification:

435 6

Abstract:

A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fixing the nucleic acids with 10% acetic acid for about 20 minutes, washing the gel multiple times with water for about 2 minutes, impregnating the gel with silver nitrate at a concentration of about 1. 0 g/l and about 1. 5 ml/l of 37% formaldehyde for about 30 minutes, developing the gel with sodium carbonate at a concentration of about 30 g/l, 1. 5 ml/l of 37% formaldehyde and sodium thiosulfate pentahydrate at a concentration of about 2. 0 mg/l for between about 2 minutes and about 5 minutes, and then stopping the development of the gel by treatment with 10% acetic acid for about 5 minutes.

US Patent:

55675859, Oct 22, 1996

Filed:

Jun 9, 1994

Appl. No.:

8/258553

Inventors:

Gustavo Caetano-Anolles - Knoxville TN
Brant J. Bassam - The University of Queensland, AU
Peter M. Gresshoff - Knoxville TN

Assignee:

The University of Tennessee Research Corporation - Knoxville TN

International Classification:

C12Q 168
G01N 2726

US Classification:

435 6

Abstract:

A silver staining method is provided for staining an organic molecule capable of binding silver. An improved image is developed and visualized. A kit useful in practicing the method is described. A permanent record of the image of the profile of the stained molecules is obtained.

US Patent:

60748184, Jun 13, 2000

Filed:

Oct 20, 1993

Appl. No.:

8/139459

Inventors:

Gustavo Caetano-Anolles - Knoxville TN
Brant J. Bassam - The University of Queensland, AU
Peter M. Gresshoff - Knoxville TN

Assignee:

The University of Tennessee Research Corporation - Knoxville TN

International Classification:

C12Q 168
C07H 2100

US Classification:

435 6

Abstract:

Improvements to nucleic acid fingerprinting are disclosed. The method generates profiles of increased fidelity characteristic of the nucleic acids analyzed. Novel primers and other means are taught. Useful products are disclosed.

US Patent:

54139090, May 9, 1995

Filed:

Jan 19, 1993

Appl. No.:

8/006380

Inventors:

Brant J. Bassam - Knoxville TN
Gustavo Caetano-Anolles - Knoxville TN
Peter M. Gresshoff - Knoxville TN

Assignee:

The University of Tennessee Research Corp. - Knoxville TN

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A method for amplifying nucleic acid sequences in a template comprising at least one nucleic acid or mixture of nucleic acids wherein each nucleic acid comprises at least one strand. The method comprises the steps of treating the template with at least one oligonucleotide primer having a nucleic acid sequence that is substantially complementary to at least one nucleic acid sequence on the template under conditions such that the primer will bind to a segment that is substantially complementary to the primer, producing primed strands of nucleic acid. The primed strands produce extension strands, wherein the extension strands comprise the primer in combination with a sequence of nucleic acids that is substantially complementary to the template in a 5' to 3' direction from the primer. The extension strands are further treated with the primer producing amplification strands which are treated with primer. The amplification strands are repeatedly treated with primer to produce large numbers of amplification strands whereby the nucleic acid sequences of the amplification strands are substantially similar to nucleic acid sequences in the original template.