Paul J Mcewan

US Patent:

20020094519, Jul 18, 2002

Filed:

Mar 7, 2002

Appl. No.:

10/092825

Inventors:

Kevin McKernan - Marblehead MA, US
Joel Malek - Beverly MA, US
Paul McEwan - Cambridge MA, US

Assignee:

AGENCOURT BIOSCIENCE CORPORATION

International Classification:

C12Q001/00
C12Q001/68

US Classification:

435/004000, 435/006000

Abstract:

The present invention provides methods for determining the sequence of nucleic acids encoding interacting polypeptide sequences. Two hybrid assays are carried out to select host cells containing nucleotide sequences encoding interacting proteins. The identity of the nucleotide sequences is determined by isolating the nucleotide sequences from the selected host cells and carrying out sequencing reactions on the nucleotide sequences.

US Patent:

20030235839, Dec 25, 2003

Filed:

Jan 16, 2003

Appl. No.:

10/346714

Inventors:

Kevin McKernan - Cambridge MA, US
Paul McEwan - Cambridge MA, US
William Morris - Cleveland Hts OH, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/025400

Abstract:

Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate.

US Patent:

20060003357, Jan 5, 2006

Filed:

May 13, 2005

Appl. No.:

11/129218

Inventors:

Kevin McKernan - Cambridge MA, US
Paul McEwan - Cambridge MA, US
William Morris - Cleveland Hts. OH, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q 1/68
C12N 1/08

US Classification:

435006000, 435270000

Abstract:

A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.

US Patent:

20100121044, May 13, 2010

Filed:

Jun 24, 2009

Appl. No.:

12/490674

Inventors:

Kevin McKernan - Cambridge MA, US
Paul McEwan - Cambridge MA, US
William Morris - Cleveland Hts. OH, US

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C07H 1/06

US Classification:

536 254

Abstract:

A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.

US Patent:

6534262, Mar 18, 2003

Filed:

May 13, 1999

Appl. No.:

09/311317

Inventors:

Kevin McKernan - Cambridge MA
Paul McEwan - Cambridge MA
William Morris - Cleveland Hts. OH

Assignee:

Whitehead Institute for Biomedical Research - Cambridge MA

International Classification:

C12Q 168

US Classification:

435 6, 435 75, 435 794, 536 254

Abstract:

A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.

US Patent:

20220380823, Dec 1, 2022

Filed:

Jul 19, 2022

Appl. No.:

17/813364

Inventors:

- Wilmington MA, US
Paul McEwan - Diablo CA, US
Martin Ranik - Santa Clara CA, US
Marliz Iain McAllister Strydom - Cape Town, ZA
Eric van der Walt - Cape Town, ZA
Ross Wadsworth - Cakeside, ZA

International Classification:

C12P 19/34
C12Q 1/6853
C12Q 1/686

Abstract:

The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5′ to 3′, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.

US Patent:

20210317422, Oct 14, 2021

Filed:

Mar 11, 2021

Appl. No.:

17/199343

Inventors:

- Wilmington MA, US
Paul J. McEwan - Diablo CA, US
Eric van der Walt - Cape Town, ZA
John Foskett - Boulder CO, US
William Bourn - Plumstead, ZA

International Classification:

C12N 9/12
C12P 19/34
C12Q 1/686

Abstract:

The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

US Patent:

20200291440, Sep 17, 2020

Filed:

Jun 4, 2020

Appl. No.:

16/892541

Inventors:

- Wilmington MA, US
Paul McEwan - Diablo CA, US
Martin Ranik - Santa Clara CA, US
Marliz Strydom - Cape Town, ZA
Eric van der Walt - Cape Town, ZA
Ross Wadsworth - Cape Town, ZA

International Classification:

C12P 19/34
C12Q 1/6853
C12Q 1/686

Abstract:

The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5′ to 3′, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.