Patrick G Halbur

US Patent:

6592873, Jul 15, 2003

Filed:

Sep 1, 1994

Appl. No.:

08/301435

Inventors:

Prem S. Paul - Ames IA
Xiang-Jin Meng - Ames IA
Patrick Halbur - Ames IA
Igor Morozov - Ames IA
Melissa A. Lum - Mendota Heights MN

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA
American Cyanamid Company - Madison NJ

International Classification:

A61K 3912

US Classification:

4242181, 424 931, 424 936, 4241861, 435 693, 4352351, 536 2372

Abstract:

The present invention provides a purified preparation containing a polynucleic acid encoding at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORFs) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), proteins at least 80% but less than 100% homologous with those encoded by one or more of ORF 2, ORF 3, ORF 4 and ORF 5 of an Iowa strain of PRRSV, proteins at least 97% but less than 100% homologous with proteins encoded by one or both of ORF 6 and ORF 7 of an Iowa strain of PRRSV, antigenic regions of such proteins which are at least 5 amino acids in length and which effectively stimulate immunological protection in a porcine host against a subsequent challenge with a PRRSV isolate, and combinations thereof, in which amino acids non-essential for antigenicity may be conservatively substituted. The present invention also concerns a polypeptide encoded by such a polynucleic acid; a vaccine comprising an effective amount of such a polynucleic acid or protein; antibodies which specifically bind to such a polynucleic acid or protein; methods of producing the same; and methods of raising an effective immunological response against a PRRSV, treating a pig infected by a PRRSV, and detecting a PRRSV.

US Patent:

7223854, May 29, 2007

Filed:

Mar 19, 2001

Appl. No.:

09/810501

Inventors:

Prem S. Paul - Ames IA, US
Xiang-Jin Meng - Ames IA, US
Patrick Halbur - Ames IA, US
Igor Morozov - Ames IA, US
Melissa A. Lum - Mendota Heights MN, US

Assignee:

Wyeth Holdings Corporation - Madison NJ
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

C07H 21/04
C12P 19/34

US Classification:

536 2372, 536 2433, 435 912, 435 9133

Abstract:

The present invention provides a purified preparation containing a polynucleic acid encoding at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORF's) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), proteins at least 80% but less than 100% homologous with those encoded by one or more of ORF 2, ORF 3, ORF 4 and ORF 5 of an Iowa strain of PRRSV, proteins at least 97% but less than 100% homologous with proteins encoded by one or both of ORF 6 and ORF 7 of an Iowa strain of PRRSV, antigenic regions of such proteins which are at least 5 amino acids in length and which effectively stimulate immunological protection in a porcine host against a subsequent challenge with a PRRSV isolate, and combinations thereof, in which amino acids non-essential for antigenicity may be conservatively substituted. The present invention also concerns a polypeptide encoded by such a polynucleic acid; a vaccine comprising an effective amount of such a polynucleic acid or protein; antibodies which specifically bind to such a polynucleic acid or protein; methods of producing the same; and methods of raising an effective immunological response against a PRRSV, treating a pig infected by a PRRSV, and detecting a PRRSV.

US Patent:

7264957, Sep 4, 2007

Filed:

Jul 15, 2003

Appl. No.:

10/618779

Inventors:

Prem S. Paul - Ames IA, US
Xiang-Jin Meng - Ames IA, US
Patrick Halbur - Ames IA, US
Igor Morozov - Ames IA, US
Melissa A. Lum - Mendota Heights MN, US

Assignee:

Wyeth - Madison NJ
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

C12N 7/00

US Classification:

4352351

Abstract:

The present invention provides a purified preparation containing a polynucleic acid encoding at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORF's) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), proteins at least 80% but less than 100% homologous with those encoded by one or more of ORF 2, ORF 3, ORF 4 and ORF 5 of an Iowa strain of PRRSV, proteins at least 97% but less than 100% homologous with proteins encoded by one or both of ORF 6 and ORF 7 of an Iowa strain of PRRSV, antigenic regions of such proteins which are at least 5 amino acids in length and which effectively stimulate immunological protection in a porcine host against a subsequent challenge with a PRRSV isolate, and combinations thereof, in which amino acids non-essential for antigenicity may be conservatively substituted. The present invention also concerns a polypeptide encoded by such a polynucleic acid; a vaccine comprising an effective amount of such a polynucleic acid or protein; antibodies which specifically bind to such a polynucleic acid or protein; methods of producing the same; and methods of raising an effective immunological response against a PRRSV, treating a pig infected by a PRRSV, and detecting a PRRSV.

US Patent:

7276353, Oct 2, 2007

Filed:

Dec 9, 2002

Appl. No.:

10/314512

Inventors:

Xiang-Jin Meng - Blacksburg VA, US
Martijn Fenaux - Blacksburg VA, US
Patrick G. Halbur - Ames IA, US

Assignee:

Virginia Tech Intellectual Properties, Inc. - Blacksburg VA
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

C12N 15/09
A61K 39/12

US Classification:

435 691, 435 693, 4241991

Abstract:

The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products.

US Patent:

7279166, Oct 9, 2007

Filed:

Mar 25, 2004

Appl. No.:

10/808964

Inventors:

Xiang-Jin Meng - Blacksburg VA, US
Martijn Fenaux - Redwood City CA, US
Patrick G. Halbur - Ames IA, US

Assignee:

Virginia Tech Intellectual Properties, Inc. - Blacksburg VA
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

A61K 39/12
C12N 15/09

US Classification:

4241991, 4242041, 536 231, 536 2372, 435 691, 435440, 435455

Abstract:

The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products. In addition, the invention encompasses two mutations in the PCV2 immunogenic capsid gene and protein, and the introduction of the ORF2 mutations in the chimeric clones.

US Patent:

7517976, Apr 14, 2009

Filed:

Mar 8, 2006

Appl. No.:

11/369944

Inventors:

Prem S. Paul - Ames IA, US
Xiang-Jin Meng - Ames IA, US
Patrick Halbur - Ames IA, US
Igor Morozov - Ames IA, US
Melissa A. Lum - Mendota Heights MN, US

Assignee:

Iowa State University Research Foundation, Inc. - Ames IA
Wyeth Holding Corporation - Madison NJ

International Classification:

C07H 21/00
C12N 1/00
C12N 5/00
C12N 15/00
C12P 1/00
C12N 15/40

US Classification:

536 2372, 435 41, 435 693, 4352541, 4353201, 435325

Abstract:

The present invention provides a purified preparation containing a polynucleic acid encoding at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORF's) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), proteins at least 80% but less than 100% homologous with those encoded by one or more of ORF 2, ORF 3, ORF 4 and ORF 5 of an Iowa strain of PRRSV, proteins at least 97% but less than 100% homologous with proteins encoded by one or both of ORF 6 and ORF 7 of an Iowa strain of PRRSV, antigenic regions of such proteins which are at least 5 amino acids in length and which effectively stimulate immunological protection in a porcine host against a subsequent challenge with a PRRSV isolate, and combinations thereof, in which amino acids non-essential for antigenicity may be conservatively substituted. The present invention also concerns a polypeptide encoded by such a polynucleic acid; a vaccine comprising an effective amount of such a polynucleic acid or protein; antibodies which specifically bind to such a polynucleic acid or protein; methods of producing the same; and methods of raising an effective immunological response against a PRRSV, treating a pig infected by a PRRSV, and detecting a PRRSV.

US Patent:

7575752, Aug 18, 2009

Filed:

Aug 15, 2007

Appl. No.:

11/893182

Inventors:

Xiang-Jin Meng - Blacksburg VA, US
Martijn Fenaux - Redwood City CA, US
Patrick G. Halbur - Ames IA, US

Assignee:

Virginia Tech Intellectual Properties, Inc. - Blacksburg VA
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

A61K 39/12
C12N 15/09

US Classification:

4241991, 4242041, 536 231, 536 2372, 435 691, 435440, 435455

Abstract:

The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products. In addition, the invention encompasses two mutations in the PCV2 immunogenic capsid gene and protein, and the introduction of the ORF2 mutations in the chimeric clones.

US Patent:

8058048, Nov 15, 2011

Filed:

Aug 11, 2009

Appl. No.:

12/539540

Inventors:

Xiang-Jin Meng - Blacksburg VA, US
Martijn Fenaux - Redwood City CA, US
Patrick G. Halbur - Ames IA, US

Assignee:

Virginia Tech Intellectual Properties, Inc. - Blacksburg VA
Iowa State University Research Foundation, Inc. - Ames IA

International Classification:

A61K 39/295
C12N 7/00

US Classification:

4352351, 4242021

Abstract:

The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2. The new chimeric infectious DNA clone and its derived, avirulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products. In addition, the invention encompasses two mutations in the PCV2 immunogenic capsid gene and protein, and the introduction of the ORF2 mutations in the chimeric clones.