Nicola L Pohl from 1214 Florida Ave, Ames, IA 50014, age 56, Phone: 515 292-7150

Name / Title

Company / Classification

Phones & Addresses

Nicola Pohl
President

Lucella Biosciences Incorporated
Business Consulting Services

1030 Roy J Carver, Ames, IA 50011

Nicola L. Pohl
President

Lucella Biosciences Incorporated
Business Consulting

1030 Roy J Carver, Ames, IA 50011

Methods For Making Polyketides
view source
US Patent:

6551802, Apr 22, 2003
Filed:

Dec 12, 2000
Appl. No.:

09/735685
Inventors:

Chaitan Khosla - Palo Alto CA
Janice Lau - Stanford CA
Nicola L. Pohl - Stanford CA
Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA
International Classification:

C12P 1962
US Classification:

435 76, 435183
Abstract:

The stereochemical centers of a polyketide can be changed by replacement of ketosynthase domains in the polyketide synthase (PKS) enzyme that produces the polyketide. The specificity of the AT domains of a PKS is determined by a hypervariable region that can be replaced or altered to change the specificity of the AT domain from a naturally occurring extender unit to another naturally or non-naturally occurring extender unit. Non-naturally occurring extender units, including methylmalonyl N-acetyl cysteamine thioester can be incorporated into polyketides in recombinant host cells or in cell-free systems to make polyketides.

More Stable Analog Of Isopropyl Thiogalactoside For Induction Of Protein Expression
view source
US Patent:

7141394, Nov 28, 2006
Filed:

Apr 19, 2004
Appl. No.:

10/827213
Inventors:

Nicola Lucia Pohl - Ames IA, US
Assignee:

Iowa State University Research Foundation, Inc. - Ames IA
International Classification:

C12P 21/04
C07H 7/02
US Classification:

435 701, 435 711, 536 111, 536124
Abstract:

A novel C-glycoside of isopropylthiogalactoside (IPTG), isobutyl-C-galactoside (IBCG), is described. IBCG may be used as an IPTG substitute for increased induction of protein expression of plasmid-based genes for the production of recombinant proteins under the control of the lac promoter. IBCG offers the advantage over IPTG of being stable at ambient temperature.

Colorimetric Monitoring Of Reactions In Solution And Solid Phase Automated And Manual Systems
view source
US Patent:

7842511, Nov 30, 2010
Filed:

Apr 13, 2006
Appl. No.:

11/403381
Inventors:

Nicola Lucia Pohl - Ames IA, US
Assignee:

Iowa State University Research Foundation, Inc. - Ames IA
International Classification:

G01N 33/00
US Classification:

436166, 436164, 436800, 422 8206, 422 8205
Abstract:

The present invention relates to the a new protecting group, 4-(O-nitrophthalimido)butyric acid (NPB) that may be attached to a monomer unit during oligosaccharide synthesis such that upon cleavage of this molecule, the solution turns orange. The orange color allows the simple colorimetric monitoring of the completion of the cleavage reaction and, upon collection of the colored fraction, quantification of the amount of the monomer unit that was successfully coupled to the solid phase.

Methods For Making Polyketides
view source
US Patent:

20040018597, Jan 29, 2004
Filed:

Mar 25, 2003
Appl. No.:

10/400092
Inventors:

Chaitan Khosla - Palo Alto CA, US
Janice Lau - Stanford CA, US
Nicola Pohl - Menlo Park CA, US
International Classification:

C12P019/60
C12N009/10
C12N001/21
C12N015/74
US Classification:

435/075000, 435/193000, 435/320100, 435/252300, 435/069100
Abstract:

The stereochemical centers of a polyketide can be changed by replacement of ketosynthase domains in the polyketide synthase (PKS) enzyme that produces the polyketide. The specificity of the AT domains of a PKS is determined by a hypervariable region that can be replaced or altered to change the specificity of the AT domain from a naturally occurring extender unit to another naturally or non-naturally occurring extender unit. Non-naturally occurring extender units, including methylmalonyl N-acetyl cysteamine thioester can be incorporated into polyketides in recombinant host cells or in cell-free systems to make polyketides.

Fluorous-Based Microarrays
view source
US Patent:

20060199211, Sep 7, 2006
Filed:

Mar 3, 2006
Appl. No.:

11/368045
Inventors:

Nicola Pohl - Ames IA, US
International Classification:

C12Q 1/68
G01N 33/53
C12M 1/34
US Classification:

435006000, 435007100, 435287200
Abstract:

A novel means of arraying fluorous tagged probes is disclosed. The method involves fluorous-tagging probes and attaching the same to a substrate having a fluorous surface. Target molecules are then identified and analyzed following attachment to the microarray.

Automated Solution-Phase Iterative Synthesis
view source
US Patent:

20100159604, Jun 24, 2010
Filed:

Jun 22, 2007
Appl. No.:

11/767098
Inventors:

Nicola Lucia Pohl - Ames IA, US
Gisun Park - Ames IA, US
Assignee:

IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. - Ames IA
International Classification:

G01N 37/00
B01J 19/00
B01J 3/00
F28D 21/00
US Classification:

436 56, 422129, 422198, 422242
Abstract:

The first method for iterative solution-phase biomolecule synthesis is described. The method requires only 3 or fewer equivalents of building block at each coupling cycle, and incorporates a FSPE step at the end of each coupling/deprotection sequence to eliminate most byproducts.

Automated Solution-Phase Iterative Synthesis
view source
US Patent:

20110009612, Jan 13, 2011
Filed:

Sep 21, 2010
Appl. No.:

12/886644
Inventors:

NICOLA LUCIA POHL - AMES IA, US
GISUN PARK - AMES IA, US
Assignee:

IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. - AMES IA
International Classification:

C08B 37/00
C07H 1/00
US Classification:

5361231
Abstract:

The first method for iterative solution-phase biomolecule synthesis is described. The method requires only 3 or fewer equivalents of building block at each coupling cycle, and incorporates a FSPE step at the end of each coupling/deprotection sequence to eliminate most byproducts.

Method For Making Polyketides
view source
US Patent:

62216414, Apr 24, 2001
Filed:

Jul 2, 1999
Appl. No.:

9/346860
Inventors:

Chaitan Khosla - Stanford CA
Janice Lau - Stanford CA
Nicola L. Pohl - Menlo Park CA
Assignee:

Leland Stanford Junior University - Palo Alto CA
International Classification:

C12N 910
US Classification:

435193
Abstract:

The stereochemical centers of a polyketide can be changed by replacement of ketosynthase domains in the polyketide synthase (PKS) enzyme that produces the polyketide. The specificity of the AT domains of a PKS is determined by a hypervariable region that can be replaced or altered to change the specificity of the AT domain from a naturally occurring extender unit to another naturally or non-naturally occurring extender unit. Non-naturally occurring extender units, including methylmalonyl N-acetyl cysteamine thioester can be incorporated into polyketides in recombinant host cells or in cell-free systems to make polyketides.