Nicholas G Theodorakis

Technical Services Consultant At Roche Applied Science (For Adecco)

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Position:

Technical Services Consultant at Roche Applied Science, Adjunct Faculty at Ivy Tech Community College

Location:

Indianapolis, Indiana Area

Industry:

Biotechnology

Work:

Roche Applied Science since Sep 2010
Technical Services Consultant
Ivy Tech Community College since Mar 2010
Adjunct Faculty
Indiana University Medical School Aug 2004 - Jul 2009
Research Assistant Professor
University of Rochester Medical School Aug 1999 - Jul 2004
Assistant Professor
Georgetown University Medical Center Dec 1998 - Jul 1999
Research Assistant Professor

Education:

Northwestern University 1983 - 1989
Ph.D, Biochemistry and Molecular and Cellular Biology; Biology Washington University in St. Louis 1978 - 1982
B.A., Biology

Skills:

Cell Biology
Molecular Biology
Clinical Research
Biochemistry
Research
In Vitro
Cancer
Immunology
Laboratory
Lifesciences

Nicholas Theodorakis Fishers, IN

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Work:

Roche Applied Science

Aug 2010 to Dec 2012
Technical Support Consultant Ivy Tech Community College
Indianapolis, IN
Mar 2010 to Dec 2010
Adjunct Professor Indiana University Medical School
Indianapolis, IN
Aug 2004 to Jul 2009
Research Assistant Professor University of Rochester Medical School
Rochester, NY
Aug 1999 to Jul 2004
Assistant Professor Georgetown University Medical Center
Washington, DC
Dec 1998 to Jul 1999
Research Assistant Professor Dept. of Pediatric Surgery, Johns Hopkins University Medical Center
Baltimore, MD
Jan 1996 to Nov 1998
Post-doctoral fellow Dept. of Biological Chemistry, Johns Hopkins University Medical Center
Baltimore, MD
May 1989 to Dec 1995
Post-doctoral fellow

Education:

Northwestern University
Evanston, IL
May 1989
Ph.D. in Biochemistry and Molecular and Cellular Biology Washington University in St Louis. St. Louis
St. Louis, MO
May 1982
Ph.D. in Biology

Skills:

Skills Communicative Technical support: providing application support and troubleshooting Teaching (medical students, undergraduates, adult education) Preparation and submission of manuscripts for publication in scientific journals Presentation of data in scientific conferences and local informal settings Maintaining collaborations with researchers within the same department as well as with those in other universities Technical Bench research using biochemical, molecular biological, and cell biological techniques to examine gene expression and biological markers using in vitro and small animal systems. Specific techniques used include (but not limited to): o Isolation of RNA from cells and tissues; analysis of RNA by RT-PCR, RNase protection, Nuclease S1 protection, or Northern blot o Cell culture of primary and established cell lines; isolation of primary cells; transfection of cells with plasmids or siRNA, immunofluorescence microscopy o Analysis of proteins by one or two-dimensional SDS-PAGE; Western blotting, enzymatic assays, ELISA, zymography, chromatography, and binding studies o Examination of transcriptional activity by nuclear run-off analysis, electrophoretic mobility shift assay (EMSA), or transfection of reporter genes o Construction and isolation of plasmid vectors used for gene expression studies or probes