Natalia A Rodionova

US Patent:

20080026373, Jan 31, 2008

Filed:

Jul 26, 2006

Appl. No.:

11/460238

Inventors:

Natalia A. Rodionova - Waltham MA, US

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435 6, 4352872

Abstract:

Assays based on probes attached to surfaces enclosed within cassettes and cassettes and reading stations for the assays. After liquids flow over the probe or probe array to form an array of photo-emissive analyte complexes, and prior to reading, (a) liquid is removed by flow, and (b) a drying gas stream, is forced into the cassette and over the complexes for a drying interval to remove liquid residue. Heating assists the drying. Light from the analyte complexes is then read through a window of the cassette. The interval of drying may be of the order of about one minute. During a preceding wash phase, gas flow bursts through the gas inlet channel purge liquid contaminant. The probes e.g. may be oligonucleotides, peptides, polypeptides, proteins, antibodies, or small molecules (steroids, expression regulators, e.g. siRNA, or other ligands). A cassette has a passage leading from a bubble removal system to the probe-bearing surface and the desiccating gas stream is introduced to that passage. For wide arrays the common passage connects through a widening transition to a wide reaction chamber. A reader station includes an air pump and liquid pumping devices such as linear actuators to deflect liquid-pumping diaphragms of the cassette.

US Patent:

20110207621, Aug 25, 2011

Filed:

Feb 20, 2009

Appl. No.:

12/918757

Inventors:

Jean I. Montagu - Brookline MA, US
Herman Deweerd - Bedford MA, US
Natalia A. Rodionova - Framingham MA, US
Nathan Tyburczy - Billerica MA, US

Assignee:

AVANTRA BIOSCIENCES CORPORATION - Boston MA

International Classification:

C40B 30/04
C40B 60/12
C40B 30/00
C40B 50/00
F04B 43/00

US Classification:

506 9, 506 39, 506 7, 506 23, 417 53

Abstract:

Flow-through assay reaction chamber () of cassette has back and forth liquid mixing in narrow gap (G) over array of capture agent (S), with net flow advance to waste confinement (), produced by reversible pumps ( or ), operable with rolling diaphragm action with at least limited elastic recovery that advance sample or buffer liquids through conditioning paths (A, ) before reaching the reaction chamber (). A single pump produces accurate flow control, liquid conditioning, e.g., liquefying dry reagent from internal surfaces of flow-dividing material (A, A′, e.g. open cell foam or frit), heating (A), and air bubble removal (), as well as replenishment of reagent while accomplishing mixing within the flow-through reaction chamber (). Lower viscosity buffer liquid is arranged to propel higher viscosity reagent, the flow-dividing storage material preserving reagent concentration. A blister pack () acts as a reversible pump () in producing accurate forward and backward flows with the net flow advance. Cascaded bubble traps () on the cassette render the system tolerant of minor pumping error during cassette priming.

US Patent:

20110319279, Dec 29, 2011

Filed:

Sep 7, 2010

Appl. No.:

12/876883

Inventors:

Jean I. Montagu - Brookline MA, US
Herman Deweerd - Bedford MA, US
Roger Dowd - Natick MA, US
Natalia Rodionova - Waltham MA, US
Peter Maimonis - Westwood MA, US
Nathan Tyburczy - Arlington MA, US

Assignee:

AVANTRA BIOSCIENCES CORPORATION - Woburn MA

International Classification:

C40B 30/04
C40B 40/10

US Classification:

506 9, 506 18

Abstract:

Cassette () performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (), flowing over wide array () of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10to 5×10, are heated in region () preceding and including bubble removal system (). Analyte is introduced through compressed septum (). External actuations of displacement pumps () and valves ( A, B, and C) produce flows in response to flow-front optical sensors (). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage () to reaction () to waste (), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.

US Patent:

20060275852, Dec 7, 2006

Filed:

Oct 27, 2005

Appl. No.:

11/262115

Inventors:

Jean Montagu - Brookline MA, US
Herman Deweerd - Bedford MA, US
Roger Dowd - Natick MA, US
Natalia Rodionova - Waltham MA, US
Peter Maimonis - Westwood MA, US
Nathan Tyburczy - Arlington MA, US

International Classification:

G01N 33/53
C12M 1/34

US Classification:

435007930, 435287200

Abstract:

Cassette () performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (), flowing over wide array () of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10to 5×10, are heated in region () preceding and including bubble removal system (). Analyte is introduced through compressed septum (). External actuations of displacement pumps () and valves (A, B, and C) produce flows in response to flow-front optical sensors (). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage () to reaction () to waste (), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.

US Patent:

20190345477, Nov 14, 2019

Filed:

May 23, 2019

Appl. No.:

16/421459

Inventors:

- Wilbraham MA, US
Kedar C. Chitale - Vernon CT, US
Krishna N. Kumar - Wilbraham MA, US
Ruan Zhang - Winchester MA, US
Benjamin Ross-Johnsrud - Northampton MA, US
Rudolf Gilmanshin - Framingham MA, US
Natalia Rodionova - Framingham MA, US
Rui Tostoes - Northampton MA, US

International Classification:

C12N 13/00
B01D 21/28
G01N 15/14
A61M 1/36
B01L 3/00
C12M 1/00

Abstract:

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

US Patent:

20180104620, Apr 19, 2018

Filed:

Oct 19, 2017

Appl. No.:

15/788784

Inventors:

- Wilbraham MA, US
Rudolf Gilmanshin - Framingham MA, US
Natalia Rodionova - Framingham MA, US
Benjamin Ross-Johnsrud - Northampton MA, US

International Classification:

B01D 15/38
C07K 17/10
C12N 13/00
C07K 1/14
C12N 1/02
B01D 21/28

Abstract:

Beads with functionalized material applied to them are exposed to an acoustic field to trap or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes.